A new intracellular bacterium, Candidatus Similichlamydia labri sp. nov. (Chlamydiaceae) producing epitheliocysts in ballan wrasse, Labrus bergylta (Pisces, Labridae)

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• 作者：Andreas Steigen ; Egil Karlsbakk ; Heidrun Plarre…
• 关键词：Epitheliocystis ; Chlamydiales ; Actinochlamydiaceae ; Similichlamydia labri ; Labridae ; Labrus bergylta
• 刊名：Archives of Microbiology
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：197
• 期：2
• 页码：311-318
• 全文大小：1,798 KB
• 参考文献：1. Bush RM, Everett KDE (2001) Molecular evolution of the Chlamydiaceae. Int J Syst Evol Microbiol 51:203-20
  2. Corsaro D, Work TM (2012) / Candidatus Renichlamydia lutjani, a gram-negative bacterium in internal organs of blue-striped snapper / Lutjanus kasmira from Hawaii. Dis Aquat Org 98:249-54 CrossRef
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  4. Draghi A, Vsevolod LP, Kahl MM, Stanton JB, Brown CC, Tsongalis G, West AB, Frasca S (2004) Characterization of -em class="a-plus-plus">Candidatus Piscichlamydia salmonis-(order Chlamydiales), a Chlamydia-like bacterium associated with epitheliocystis in farmed Atlantic salmon ( / Salmo salar). J Clin Microbiol 42:5286-297 CrossRef
  5. Draghi A, Bebak J, Popov VL, Noble AC, Geary SJ, West AB, Byrne P, Frasca S (2007) Characterization of a / Neochlamydia-like bacterium associated with epitheliocystis in cultured Arctic charr / Salvelinus alpinus. Dis Aquat Org 76:27-8 CrossRef
  6. Everett KD (2000) / Chlamydia and / Chlamydiales: more than meets the eye. Vet Microbiol 75:109-26 CrossRef
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  8. Heuch PA, Bj?rn PA, Finstad B, Holst JC, Asplin L, Nilsen F (2005) A review of the Norwegian ‘National action plan against salmon lice on salmonids- the effect on wild salmonids. Aquaculture 246:79-2 CrossRef
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  10. Karlsen M, Nylund A, Watanabe K, Helvik JV, Nylund S, Plarre H (2008) Characterization of / Candidatus Clavochlamydia salmonicola: an intracellular bacterium infecting salmonid fish. Environ Microbiol 10:208-18
  11. Kim M, Oh H-S, Park S-C, Chun J (2014) Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Intern J Syst Evol Microbiol 64:346-51 CrossRef
  12. Meijer A, Roholl PJM, Ossewaarde JM, Jones B, Nowak BF (2006) Molecular evidence for association of / Chlamydiales bacteria with epitheliocystis in leafy seadragon ( / Phycodurus eques), silver perch ( / Bidyanus bidyanus), and barramundi ( / Lates calcarifer). Appl Environ Microbiol 72:284-90 CrossRef
  13. Mendoza M, Guiza L, Martinez X, Caraballo X, Rojas J, Aranguren LF, Salazar M (2013) A novel agent ( / Endozoicomonas elysicola) responsible for epitheliocystis in cobia / Rachycentrum canadum larvae. Dis Aquat Org 106:31-7 CrossRef
  14. Mitchell SO, Steinum TM, Toenshoff ER, Kvellestad A, Falk K, Horn M, Colquhoun DJ (2013) / Candidatus Branchiomonas cysticola is a common agent of epitheliocystis in seawater-farmed Atlantic salmon in Norway and Ireland. Dis Aquat Org 103:35-3 CrossRef
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• 作者单位：Andreas Steigen (1)
  Egil Karlsbakk (1) (2)
  Heidrun Plarre (1)
  Kuninori Watanabe (1)
  Aina-Cathrine ?verg?rd (3)
  ?yvind Brevik (1)
  Are Nylund (1)
  
  1. Department of Biology, University of Bergen, Bergen, Norway
  2. Institute of Marine Research, Bergen, Norway
  3. Sea Lice Research Center (SLRC), Institute of Marine Research, 5817, Bergen, Norway
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Microbial Ecology
  Biochemistry
  Cell Biology
  Biotechnology
  Ecology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-072X

文摘

Certain wrasse species (Labridae) are used as cleaner fish in salmon farms on the Norwegian coast, reducing salmon louse intensities. The pathogen repertoire of wrasse in Norway is poorly known, and the objective of the present study is to describe a novel intracellular bacterium detected in Norwegian Labrus bergylta. Histological examination of gill tissues from ballan wrasse, L. bergylta, revealed epitheliocysts occurring basally to the secondary lamellae in the interlamellar epithelium. Ultrastructurally, these had bacteria-filled inclusions with thickened membranes and radiating ray-like structures (actinae). 16S rRNA gene sequences from the gill bacteria showed the highest (97.1?%) similarity to Candidatus Similichlamydia latridicola from the gills of the latrid marine fish Latris lineata in Australia and 94.9?% similarity to Candidatus Actinochlamydia clariae, causing epitheliocystis in the freshwater catfish Clarias gariepinus in Uganda. A total of 47 gill samples from L. bergylta from Western Norway were screened by real time RT-PCR with an assay targeting Candidatus Actinochlamydiaceae 16S rRNA. Prevalence was 100?%. We propose the name Candidatus Similichlamydia labri sp. nov. for this new agent producing gill epitheliocysts in L. bergylta.