Development of SNP and microsatellite markers for goldsinny wrasse (Ctenolabrus rupestris) from ddRAD sequencing data

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• 作者：E. Jansson ; J. B. Taggart ; S. Wehner ; G. Dahle…
• 关键词：SNP ; Microsatellite ; Goldsinny wrasse ; Ctenolabrus rupestris ; Cleaner fish
• 刊名：Conservation Genetics Resources
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：8
• 期：3
• 页码：201-206
• 全文大小：336 KB
• 刊物主题：Conservation Biology/Ecology; Plant Sciences; Animal Anatomy / Morphology / Histology; Evolutionary Biology;
• 出版者：Springer Netherlands
• ISSN：1877-7260
• 卷排序：8

文摘

Wrasse (Labridae) species have been used as parasite cleaners in Atlantic salmon farming since the 1980s. However, their use has recently escalated, with millions now being introduced into salmon cages each year. Most fish are of wild origin, their exploitation potentially impacting native populations. Genetic information is urgently required to inform management decisions. We identified 174 microsatellite and 149 SNP markers from ddRAD sequence data. From these, 17 and 48 microsatellite and SNP markers, respectively, were validated by genotyping 150 goldsinny wrasse collected from five locations along the Norwegian and Swedish coasts. Two to 30 alleles were identified at the microsatellite loci, while gene diversity (H_e) ranged 0.101–0.907. All SNP loci were biallelic, with averaged H_e per locus ranging between 0.063 and 0.495.