1H, 15N and 13C backbone assignments of GDP-bound human H-Ras mutant G12V

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• 作者：Nader Amin ; Elisabetta Chiarparin ; Joe Coyle…
• 关键词：Ras ; H ; Ras ; H ; Ras G12V ; Cancer ; NMR ; NMR resonance assignments
• 刊名：Biomolecular NMR Assignments
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：10
• 期：1
• 页码：121-123
• 全文大小：490 KB
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• 作者单位：Nader Amin (1) (2)
  Elisabetta Chiarparin (1) (3)
  Joe Coyle (1)
  Daniel Nietlispach (4)
  Glyn Williams (1)
  
  1. Astex Pharmaceuticals, 436 Cambridge Science Park, Cambridge, CB4 0QA, UK
  2. Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK
  3. AstraZeneca, 310 Cambridge Science Park, Cambridge, CB4 0FZ, UK
  4. Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge, CB2 1GA, UK
  
• 刊物类别：Physics and Astronomy
• 刊物主题：None Assigned
• 出版者：Springer Netherlands
• ISSN：1874-270X

文摘

Harvey Ras (H-Ras) is a membrane-associated GTPase with critical functions in cell proliferation and differentiation. The G12V mutant of H-Ras is one of the most commonly encountered oncoproteins in human cancer. This mutation disrupts the GTPase activity of H-Ras, leading to constitutive activation and aberrant downstream signalling. Here we report the backbone resonance assignments of human H-Ras mutant G12V lacking the C-terminal membrane attachment domain.