In vitro propagation, genetic and phytochemical assessment of Habenaria edgeworthii: an important Astavarga plant

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• 作者：Lalit Giri (1)
  Arun Jugran (1)
  Sandeep Rawat (1)
  Praveen Dhyani (1)
  Harish Andola (1)
  Indra D. Bhatt (1)
  Ranbeer S. Rawal (1)
  Uppeandra Dhar (2)
  
• 关键词：Orchidaceae ; Protocorm ; like bodies (PLBs) ; Inter ; simple sequence repeats (ISSR) ; Tuber induction ; Genetic stability ; Total phenolic content
• 刊名：Acta Physiologiae Plantarum
• 出版年：2012
• 出版时间：May 2012
• 年：2012
• 卷：34
• 期：3
• 页码：869-875
• 全文大小：461KB
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• 作者单位：Lalit Giri (1)
  Arun Jugran (1)
  Sandeep Rawat (1)
  Praveen Dhyani (1)
  Harish Andola (1)
  Indra D. Bhatt (1)
  Ranbeer S. Rawal (1)
  Uppeandra Dhar (2)
  
  1. G. B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, 263 643, Uttarakhand, India
  2. Department of Botany, Hamdard University (Jamia Hamdard), New Delhi, 110 062, India
  

文摘

An efficient in vitro propagation protocol for Habenaria edgeworthii Hook. f. ex. Collett using seed-derived callus was established. The maximum seed germination was observed in Murashige and Skoog (MS) medium supplemented with 1.0?μM α-naphthalene acetic acid (NAA). Induction of callus was achieved on full and ?-strength MS medium supplemented with 1.0?μM NAA. The highest number of shoot (11.9 shoots/explant) was achieved in MS medium supplemented with 0.1?μM 6-benzyladenine (BA) and 0.01?μM NAA. Further, elongated shoots when transferred to ?-strength MS rooting medium with different auxin concentrations induced roots (41.6-3.3%) and tubers (0-0.8%); however, a maximum of 87.5% rooting was achieved in a plant growth regulator (PGR)-free MS medium. Rooted shoots (plantlets) when transferred to a mixture of soil:sand:perlite (1:1:1 ratio) resulted in 68% survival. Inter-simple sequence repeats (ISSR) markers confirmed the genetic stability among regenerated plants. The phytochemical analysis of tissue culture-raised tubers showed higher phenolic content than wild tuber. The regeneration protocol developed in this study provides a basis for germplasm conservation and harnessing the total phenol and phenolic compounds of H. edgeworthii. Further, the methods can open avenues for application in other Orchidaceous plants of the Indian Himalayan region.