参考文献:1. Anjum N.A., Umar S., Ahmad A., Iqbal M. & Khan A. 2008. Sulphur protects mustard ( / Brassica campestris L.) from cadmium toxicity by improving leaf ascorbate and glutathione. Plant Growth Regul. 54: 271-79. CrossRef 2. Bauddh K. & Singh R.P. 2011. Differential toxicity of cadmium to mustard ( / Brassica juncia L.) genotypes under higher metal levels. J. Environ. Biol. 32: 355-62. 3. Blaylock M.J., Salt D.E., Dushenkov S., Zakharova O., Gussman C., Kapulink Y., Ensley B.D. & Raskin I. 1997. Enhanced accumulation of Pb in Indian mustard by soil-applied chelating agents. Environ. Sci. Technol.31: 856-60. 4. Blaylock M.J. & Huang J.W. 2000. Phytoextraction of metals, pp. 53-0. In: Raskin I. & Ensley B.D. (eds), Phytoremediation of toxic metals, Using plants to clean up the environment, John Wiley & Sons, USA. 5. Bogs J., Bourbouloux A., Cagnac O., Wachter A., Rausch T. & Delrot S. 2003. Functional characterization and expression analysis of a glutathione transporter, BjGT1, from / Brassica juncea: evidence for regulation by heavy metal exposure. Plant Cell Environ. 26: 1703-711. CrossRef 6. Brooks R.R. 1998. Plants that hyperaccumulate heavy metals. CAB International, Wallingford, 384 pp. 7. Brundrett M.C., Kendrick B. & Peterson C.A. 1991. Efficient lipid staining in plant material with sudan red 7B or fluoral yellow 088 in polyethylene glycol-glycerol. Biotech. Histochem. 66: 111-16. CrossRef 8. Cocker K.M., Evans D.E. & Hodson M.J. 1998. The amelioration of aluminium toxicity by silicon in higher plants: solution chemistry or in planta mechanism? Physiol. Plant. 104: 608-14. CrossRef 9. Datnoff L.E., Deren C.W. & Snyder G.H. 1997. Silicon fertilization for disease management of rice in Florida. Crop Protection 16: 525-31. CrossRef 10. Epstein E. 1999. Silicon. Annu Rev. Plant Physiol. Plant Mol. Biol. 50: 641-64. CrossRef 11. Ghani A. 2011. Varietal differences in canola ( / Brassica napus L.) for the growth, yield and yield components exposed to cadmium stress. J. Animal Plant Sci. 21: 57-9. 12. Gill S.S., Khan N.A. & Tuteja N. 2011. Differential cadmium stress tolerance in five indian mustard (Brassica juncea L.) cultivars, an evaluation of the role of antioxidant machinery. Plant Signal. Behav. 6: 293-00. CrossRef 13. Hodson M.J. & Evans D.E. 1995. Aluminium/silicon interactions in higher plants. J. Exp. Bot. 46: 161-71. CrossRef 14. Hodson M.J., White P.J., Mead A. & Broadley M.R. 2005. Phylogenetic variation in the silicon composition of plants. Ann. Bot. 96: 1027-046. CrossRef 15. Kumar P.B.A.N., Dushenkov V., Motto H. & Raskin I. 1995. Phytoextraction: the use of plants to remove heavy metals from soils. Environ. Sci. Technol. 29: 1232-238. CrossRef 16. Liang Y., Sun W., Zhu Y.G. & Christie P. 2007. Mechanisms of silicon-mediated alleviation of abiotic stresses in higher plants: A review. Env. Pol. 147: 422-28. CrossRef 17. Lichtenthaler H.K. 1987. Chlorophylls and carotenoids: pigments of photosynthetic membranes. Methods Enzymol. 148: 350-82. CrossRef 18. Luka?ová-Kulíková Z. & Lux A. 2010. Silicon influence on maize, / Zea mays L., hybrids exposed to cadmium treatment. Bull. Environ. Contam. Toxicol. 85: 243-50. 19. Luná?ková L., ?ottníková A., Masarovi?ová E., Lux A. & Stre?ko V. 2003. Comparison of cadmium effect on willow and poplar in response to different cultivation conditions. Biol. Plant. 47: 403-11. CrossRef 20. Lux A., Martinka M., Vaculík M. & White P.J. 2011. Root responses to cadmiumin the rhizosphere: a review. J. Exp. Bot. 62: 21-7. CrossRef 21. Lux A., Morita S., Abe J. & Ito K. 2005. An improved method for clearing and staining free-hand sections and whole-mount samples. Ann. Bot. 96: 989-96. CrossRef 22. Ma J.F. 2004. Role of silicon in enhancing the resistence of plants to biotic and abiotic stresses. Soil Sci. Plant Nutr. 50: 11-8. CrossRef 23. Ma J.F. & Takahashi E. 2002. Soil, fertilizer, and plant silicon research in Japan. Amsterdam: Elsevier Science, 294 pp. 24. Ma J.F., Tamai K., Yamaji N., Mitani N., Konishi S., Katsuhara M., Ishiguro M., Murata Y. & Yano M. 2006. A silicon transporter in rice. Nature 440: 688-91. CrossRef 25. Marchiol L., Sacco P., Assolari S. & Zerbi G. 2004. Reclamation of polluted soil: phytoremediation. Potential of crop-related / Brassica species. Water Air Soil Pollut. 158: 345-56. 26. Markovska Y.K., Gorinova N.I., Nedkovska M.P. & Miteva K.M. 2009. Cadmium-induced oxidative damage and antioxidant responses in / Brassica juncea plants. Biol. Plant. 53: 151-54. CrossRef 27. Marques A., Rangel A.O.S.S. & Castro P.M.L. 2009. Remediation of heavy metal contaminated soils: Phytoremediation as a potentially promising clean-up technology. Crit. Rev. Environ. Sci. Technol. 39: 622-54. CrossRef 28. Martinka M. & Lux A. 2004. Response of roots of three populations of / Silene dioica to cadmium treatment. Biologia 59: 185-89. 29. Masarovi?ová E., Kr?ová K., Malovcová ?., Surov?ík J., Bresti? M. & Ol?ovská K. 2008. Genotypic differences of rapeseed from the aspect of oil content, oil quality and seed production. Book of abstracts. 15. vedecká konferencia ?Nové poznatky z genetiky a ?lachtenia po?nohospodáiskych rastlín-pp. 19-2. (In Slovak) 30. McGrath S.P. 1998. Phytoextraction for soil remediation, pp. 261-88. In: Brooks R.R. (ed.), Plants that hyperaccumulate heavy metals: their role in phytoremediation, microbiology, archeology, mineral exploration and phytomining, CAN International, New York. 31. Neumann D. & zur Nieden U. 2001. Silicon and heavy metal tolerance of higher plants. Phytochemistry 56: 685-92. CrossRef 32. Nouairi I., Ammar W.B., Youssef N.B., Daoud D.B.M., Ghorbal M.H. & Zarrouk M. 2006. Comparative study of cadmium effects on membrane lipid composition of / Brassica juncea and / Brassica napus leaves. Plant Sci. 170: 511-19. CrossRef 33. Okuda A. & Takahashi E. 1965. The role of silicon, pp. 123-46. In: The Mineral Nutrition of the Rice. Symposium of the International Rice Research Institute. The John Hopkins Press, Baltimore, Maryland. 34. Pavlovi? A., Masarovi?ová E., Kr?ová K. & Kubová J. 2006. Response of chamomile plants ( / Matricaria recutita L.) to cadmium treatment. Bull. Environ. Contam. Toxicol. 77: 763-71. CrossRef 35. Pe?ko M., Kr?ová K. & Masarovi?ová E. 2011. Phytotoxic effect of some metal ions on selected rapeseed cultivars registered in Slovakia. Proceedings of ECOpole 5: 83-6. 36. Pollard A.J., Powell K.D., Harper F.A. & Smith J.A.C. 2002. The genetic basis of metal hyperaccumulation in plants. Crit. Rev. Plant Sci. 21: 539-66. CrossRef 37. Prasad M.N.V. & Freitas, H. M. O. 2003. Metal hyperaccumulation in plants -Biodiversity prospecting for phytoremediation technology. Electron. J. Biotechnol. 6: 285-21. CrossRef 38. Raskin I., Kumar P.B.A.N., Dushenkov S. & Salt D. 1994. Bioconcentration of heavy metals by plants. Curr. Opin. Biotechnol. 5: 285-90. CrossRef 39. Redjala T., Zelko I., Sterckeman T., Legué V. & Lux A. 2011. Relationship between root structure and root cadmium uptake in maize. Environ. Exp. Bot. 71: 241-48 CrossRef 40. Robinson B.H., Ba?uelos G., Conesa H.M., Evangelon W.H. & Schulin R. 2009. The phytomanagement of trace elements in soil. Crit. Rev. Plant Sci. 28: 240-66. CrossRef 41. Schreiber L., Hartmann K., Skrabs M. & Zeier J. 1999. Apoplastic barriers in roots: chemical composition of endodermal and hypodermal cell walls. J. Exp. Bot. 50: 1267-280. CrossRef 42. Song A.L., Li P., Li Z.J. & Liang Y.C. 2011. Parameters of / Brassica campestris L. ssp. / chinensis (L.) making grown under Cd stress. Acta Hort. Sin. 38: 1675-684. 43. Song. A., Li Z., Zhang J., Xue G., Fan F. & Liang Y. 2009. Silicon-enhanced resistance to cadmium toxicity in / Brassica chinensis L. is attributed to Si-suppressed cadmium uptake and transport and Si-enhanced antioxidant defense capacity. J. Hazard. Mater. 172: 74-3. CrossRef 44. ?imonová E., Henselová M., Masarovi?ová E. & Kohanová J. 2007. Comparison of tolerance of / Brassica juncea and / Vigna radiata to cadmium. Biol. Plant. 51: 488-92. CrossRef 45. Vaculík M., Lux A., Luxová M., Tanimoto E. & Lichtscheidl I. 2009. Silicon mitigates cadmium inhibitory effect in young maize plants. Environ. Exp. Bot. 67: 52-8. CrossRef 46. Vaculík M., Landberg M., Greger M., Luxová M., Stoláriková M. & Lux A. 2012. Silicon modifies root anatomy, uptake and subcellular distribution of cadmium in young maize plants. Ann. Bot. 109: (In print) 47. Vamerali T., Bandiera M. & Mosca G. 2010. Field crops for phytoremediation of metal-contaminated land. A review. Environ. Chem. Lett. 8: 1-7. CrossRef 48. Weerakoon S.R. & Somaratne S. 2009. Phytoextractive potential among mustard ( / Brassica juncea) genotypes in Sri Lanka. Cey. J. Sci. (Bio. Sci.) 38: 85-3. 49. Zelko I. & Lux A. 2004. Effect of cadmium on / Karwinskia humboldtiana roots. Biologia 59: 205-09. 50. Zelko I., Lux A., Sterckeman T., Martinka M., Kollárová K. & Li?ková D. 2012. An easy method for cutting and fluorescent staining of thin roots. Ann. Bot. 109: (In print) 51. Zhang C., Wang L., Nie Q., Zhang W. & Zhang F. 2008. Longterm effects of exogenous silicon on cadmium translocation and toxicity in rice ( / Oryza sativa L.). Environ. Exp. Bot. 62: 300-07. CrossRef 52. Zhu Y.L., Pilon-Smits E.A.H., Jouanin L. & Terry N. 1999. Overexpression of gluthathione synthetase in / Brassica juncea enhances cadmium tolerance and accumulation. Plant Physiol. 119: 73-9. CrossRef
作者单位:Zuzana Vatehová (1) (2) Karin Kollárová (1) Ivan Zelko (1) Danica Richterová-Ku?erová (1) (3) Marek Bujdo? (4) Desana Li?ková (1)
1. Department of Glycobiotechnology, Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84538, Bratislava, Slovakia 2. Department of Plant Physiology, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynská dolina, SK-84215, Bratislava, Slovakia 3. Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84523, Bratislava, Slovakia 4. Institute of Geology, Faculty of Natural Sciences, Comenius University, Mlynská dolina, SK-84215, Bratislava, Slovakia
文摘
The objective of this study was to determine the effect of silicon (Si) and cadmium (Cd) on root and shoot growth and Cd uptake in two hydroponically cultivated Brassica species (B. juncea (L.) Czern. cv. Vitasso and B. napus L. cv. Atlantic). Both species are potentially usable for phytoextraction. Inhibitory effects of Cd on root elongation were diminished by the impact of Si. Primary roots elongation in the presence of Cd + Si compared with Cd was stronger and the number of lateral roots was lower in B. juncea than in B. napus. Cd content per plant was higher in B. napus roots and shoots compared with B. juncea. Suberin lamellae were formed closer to the root apex in Cd + Si than in Cd treated plants and this effect was stronger in B. napus than in B. juncea. Accelerated maturation of endodermis was associated with reduced Cd uptake. Cd decreased the content of chlorophylls and carotenoids in both species, but Si addition positively influenced the content of photosynthetic pigments which was higher in B. napus than in B. juncea. Si enhanced more substantially translocation of Cd into the shoot of B. napus than of B. juncea. Based on our results B. napus seems to be more suitable for Cd phytoextraction than B. juncea because these plants produce more biomass and accumulate higher amount of Cd. The protective effect of Si on Cd treated Brassica plants could be attributed to more extensive development of suberin lamellae in endodermis.