Cross-platform metabolic profiling: application to the aquatic model organism Lymnaea stagnalis

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• 作者：Sara Tufi (1)
  Marja H. Lamoree (1)
  Jacob De Boer (1)
  Pim E. G. Leonards (1)
  
  1. Institute for Environmental Studies (IVM) ; VU University Amsterdam ; De Boelelaan 1087 ; 1081 HV ; Amsterdam ; The Netherlands
  
• 关键词：Metabolomics ; Lymnaea stagnalis ; Cross ; platform ; LC ; MS ; GC ; MS ; HILIC
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：407
• 期：7
• 页码：1901-1912
• 全文大小：809 KB
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Food Science
  Inorganic Chemistry
  Physical Chemistry
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1618-2650

文摘

The freshwater pond snail Lymnaea stagnalis is used in several studies on molecular and behavioral neurobiology and ecotoxicology showing its successful application as a model organism. In the present study, a cross-platform metabolomic approach has been evaluated to characterize the organ molecular phenotypes of L. stagnalis central nervous system (CNS), digestive gland (DG), and albumen gland (AG). Two types of tissue disruption methods were evaluated of which beads beating was the preferred method. To obtain a broad picture of the hydrophilic and lipophilic metabolome, two complementary analytical platforms have been employed: liquid chromatography (LC) and gas chromatography (GC) coupled to high-resolution mass spectrometry. Furthermore, to increase the power to separate small polar metabolites, hydrophilic interaction liquid chromatography (HILIC) was applied. The analytical platform performances have been evaluated based on the metabolome coverage, number of molecular features, reproducibility, and multivariate data analysis (MVDA) clustering. This multiplatform approach is a starting point for future global metabolic profiling applications on L. stagnalis.