Vertical distribution of diazotrophic bacterial community associated with temperature and oxygen gradients in a subtropical reservoir

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• 作者：Zheng Yu (1) (2)
  Jing Zhou (1)
  Jun Yang (1)
  Xiaoqing Yu (1)
  Lemian Liu (1)
  
• 关键词：nifH gene ; Cyanobacteria ; Stratified reservoir ; DGGE ; Clone library ; Quantitative real ; time PCR
• 刊名：Hydrobiologia
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：741
• 期：1
• 页码：69-77
• 全文大小：492 KB
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• 作者单位：Zheng Yu (1) (2)
  Jing Zhou (1)
  Jun Yang (1)
  Xiaoqing Yu (1)
  Lemian Liu (1)
  
  1. Aquatic EcoHealth Group, Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, People’s Republic of China
  2. University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China
  
• ISSN：1573-5117

文摘

Nitrogen-fixing microorganisms play important roles in the structure and function of aquatic ecosystems. However, the diversity and distribution of diazotrophic bacteria along the lake depth continuum are so far poorly understood. In this study, we investigated the dynamic variations of diazotrophs in a subtropical deep reservoir during the stratified period. We applied an in-depth biomolecular approach (DGGE, clone libraries, and quantitative real-time PCR) to explore the nitrogenase (nifH) gene diversity and abundance. The diazotrophic community shifted between the oxic/anoxic interface and the nifH diversity increased with depth. The Cyanobacteria, affiliated to the toxic bloom-forming Cylindrospermopsis raciborskii, were the dominant diazotrophic cluster in the surface waters, whereas diazotrophic?Alphaproteobacteria were dominant in the bottom waters. The relationships between microbial and environmental factors clearly demonstrated that the temperature gradient and the oxygen concentration affect the heterogeneity of the diazotrophic community, thereby influencing the entire aquatic nitrogen cycle.