An Environmental-Sensitive BODIPY®-Derivative with Bioapplication: Spectral and Photophysical Properties

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• 作者：Isaksson ; Mikael ; Kalinin ; Stanislav ; Lobov ; Sergei ; Ny ; Tor ; Johansson ; Lennart B. &Aring ; .
• 关键词：BODIPY ; donor– ; donor energy migration ; homotransfer ; dimers ; quenching
• 刊名：Journal of Fluorescence
• 出版年：2003
• 出版时间：2003
• 年：2003
• 卷：13
• 期：5
• 页码：379-384
• 全文大小：109 KB

文摘

A previously synthesised derivative of BODIPY aimed for sulfhydryl specific labelling of cysteine residues in proteins was studied. The spectral and photophysical properties of this derivative, N-(4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene-2-yl) iodoacetamide (NBDY) were characterised, and found to be considerably different from those of commonly used derivatives of BODIPY, e.g. N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-yl)methyl iodoacetamide. The absorption and fluorescence spectra, as well as fluorescence lifetimes and quantum yields of NBDY are quite sensitive to solvent properties. The fluorescence is effectively quenched by I⁻ when NBDY is free in water or attached to Cys in different mutants of plasminogen activator inhibitor type 2 (PAI-2). A ground-state dimer forms when two NBDY groups are closely spaced in plasminogen activator inhibitor type 1 (PAI-1).