Spectratype analysis of the T cell receptor δ CDR3 region of bovine γδ T cells responding to leptospira

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• 作者：Carolyn T. A. Herzig ; Vanessa L. Mailloux ; Cynthia L. Baldwin
• 关键词：γδ T cells ; Spectratyping ; Immunoscope ; TCR ; CDR3 ; Leptospira ; TRD
• 刊名：Immunogenetics
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：67
• 期：2
• 页码：95-109
• 全文大小：3,037 KB
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• 作者单位：Carolyn T. A. Herzig (1) (2)
  Vanessa L. Mailloux (1)
  Cynthia L. Baldwin (1)
  
  1. Department of Veterinary and Animal Sciences, University of Massachusetts, Integrated Sciences Building, 661 N. Pleasant St, Amherst, MA, 01003, USA
  2. Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, NY, 10032, USA
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Immunology
  Allergology
  Cell Biology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-1211

文摘

Gamma delta T cells comprise the majority of blood T cells in ruminants at birth and remain at high levels for several years with most expressing the WC1 co-receptor. A subpopulation of Bos taurus WC1+ cells expressing a restricted set of WC1 molecules respond immediately by proliferation and interferon-γ production to leptospira following vaccination, preceding the response by CD4 T cells. Our goal is to define the γδ T cell recognition elements involved. Previously, we showed that the responding cells employed a variety of TRDV genes indicating that the CDR1 and CDR2 of TCRδ could vary and may not be principally involved in antigen specificity. Murine and human γδ T cells bind T22 and self lipids through their CDR3δ. Like mice, cattle use up to five TRDD genes in a single CDR3δ adding flexibility to length and configuration for antigen binding. Here, we used spectratyping to evaluate the CDR3δ of leptopsira-responsive cells. Little or no compartmentalization of CDR3δ was found for antigen-responsive cells that incorporated TRDV1, TRDV2, or TRDV3 even though they comprise the majority of the leptospira-responding population. Compartmentalization occurred for TRDV4-containing transcripts and was maintained over time and among cattle. However, no common amino acid motif was apparent in those CDR3δ sequences, although a bias in D gene usage occurred. We hypothesize that the restricted set of WC1 co-receptors expressed by the responding cells may lend specificity to the response through their ability to bind bacteria facilitating interaction of various TCRs with bacterial components resulting in cross-linking and activation.