Humanizing glycosylation pathways in eukaryotic expression systems
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- 作者：Amjad Hayat Khan ; Hadi Bayat…
- 关键词：Glycosylation pattern ; Glycoengineering ; LEXSY ; Eukaryotic expression systems
- 刊名：World Journal of Microbiology & Biotechnology
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：33
- 期：1
- 全文大小：549 KB
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry
Applied Microbiology
Biotechnology
Biochemistry
Environmental Biotechnology
Microbiology - 出版者：Springer Netherlands
- ISSN：1573-0972
- 卷排序：33
文摘
Glycosylation represents the most widespread posttranslational modifications, found in a broad spectrum of natural and therapeutic recombinant proteins. It highly affects bioactivity, site-specificity, stability, solubility, immunogenicity, and serum half-life of glycoproteins. Numerous expression hosts including yeasts, insect cells, transgenic plants, and mammalian cells have been explored for synthesizing therapeutic glycoproteins. However, glycosylation profile of eukaryotic expression systems differs from human. Glycosylation strategies have been proposed for humanizing the glycosylation pathways in expression hosts which is the main theme of this review. Besides, we also highlighted the glycosylation potential of protozoan parasites by emphasizing on the mammalian-like glycosylation potential of Leishmania tarentolae known as Leishmania expression system.