Comparison of Reprogramming Genes in Induced Pluripotent Stem Cells and Nuclear Transfer Cloned Embryos
详细信息 查看全文
- 作者：Lian Duan (1)
Zhendong Wang (1)
Jingling Shen (1) (2)
Zhiyan Shan (1) (2)
Xinghui Shen (1) (2)
Yanshuang Wu (1) (2)
Ruizhen Sun (1) (2)
Tong Li (1)
Rui Yuan (1)
Qiaoshi Zhao (1)
Guangyu Bai (1)
Yanli Gu (1)
Lianhong Jin (1) (2)
Lei Lei (1) (2) - 关键词：Somatic cell nuclear transfer ; Induced pluripotent stem cells ; Reprogramming ; Gene expression profiling ; DNA microarray analysis
- 刊名：Stem Cell Reviews and Reports
- 出版年：2014
- 出版时间：August 2014
- 年：2014
- 卷：10
- 期：4
- 页码：548-560
- 全文大小：2,960 KB
- 参考文献：1. Feng, R., & Lengner, C. (2013). Application of Stem Cell Technology in Dental Regenerative Medicine. / Adv Wound Care (New Rochelle), 2(6), 296鈥?05. doi:10.1089/wound.2012.0375 . CrossRef
2. Wilmut, I., Schnieke, A. E., McWhir, J., Kind, A. J., & Campbell, K. H. (1997). Viable Offspring Derived from Fetal and Adult Mammalian Cells. / Nature, 385(6619), 810鈥?13. doi:10.1038/385810a0 . CrossRef
3. Takahashi, K., & Yamanaka, S. (2006). Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors. / Cell, 126(4), 663鈥?76. CrossRef
4. Pralong, D., Trounson, A. O., & Verma, P. J. (2006). Cell Fusion for Reprogramming Pluripotency: Toward Elimination of the Pluripotent Genome. / Stem Cell Reviews, 2(4), 331鈥?40. CrossRef
5. Xu, Y. N., Guan, N., Wang, Z. D., Shan, Z. Y., Shen, J. L., Zhang, Q. H., Jin, L. H., & Lei, L. (2009). ES Cell Extract-Induced Expression of Pluripotent Factors in Somatic Cells. / Anat Rec (Hoboken), 292(8), 1229鈥?234. doi:10.1002/ar.20919 . CrossRef
6. Fulka, J., Jr., & Fulka, H. (2007). Somatic Cell Nuclear Transfer (SCNT) in Mammals: The Cytoplast and its Reprogramming Activities. / Advances in Experimental Medicine and Biology, 591, 93鈥?02. doi:10.1007/978鈥?鈥?87鈥?7754鈥?_7 . CrossRef
7. Wakayama, S., & Wakayama, T. (2010). Improvement of Mouse Cloning Using Nuclear Transfer-Derived Embryonic Stem Cells and/or Histone Deacetylase Inhibitor. / The International Journal of Developmental Biology, 54(11鈥?2), 1641鈥?648. CrossRef
8. Teng, H. F., Kuo, Y. L., Loo, M. R., Li, C. L., Chu, T. W., Suo, H., Liu, H. S., Lin, K. H., & Chen, S. L. (2010). Valproic Acid Enhances Oct4 Promoter Activity in Myogenic Cells. / Journal of Cellular Biochemistry, 110(4), 995鈥?004. doi:10.1002/jcb.22613 . CrossRef
9. Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., & Yamanaka, S. (2007). Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors. / Cell, 131(5), 861鈥?72. CrossRef
10. Kang, L., Kou, Z., Zhang, Y., & Gao, S. (2010). Induced Pluripotent Stem Cells (iPSCs) a new era of Reprogramming. / Journal of Genetics and Genomics, 37(7), 415鈥?21. CrossRef
11. Nagata, N., & Yamanaka, S. (2014). Perspectives for Induced Pluripotent Stem Cell Technology: new Insights into Human Physiology Involved in Somatic Mosaicism. / Circulation Research, 114(3), 505鈥?10. CrossRef
12. Kishigami, S., Mizutani, E., Ohta, H., Hikichi, T., Thuan, N. V., Wakayama, S., Bui, H. T., & Wakayama, T. (2006). Significant Improvement of Mouse Cloning Technique by Treatment With Trichostatin a After Somatic Nuclear Transfer. / Biochemical and Biophysical Research Communications, 340(1), 183鈥?89. CrossRef
13. Bolstad, B. M., Irizarry, R. A., Astrand, M., & Speed, T. P. (2003). A Comparison of Normalization Methods for High Density Oligonucleotide Array Data Based on Variance and Bias. / Bioinformatics, 19(2), 185鈥?93. CrossRef
14. Tseng, G. C., Ghosh, D., & Feingold, E. (2012). Comprehensive Literature Review and Statistical Considerations for Microarray Meta-Analysis. / Nucleic Acids Research, 40(9), 3785鈥?799. CrossRef
15. Assou, S., Le Carrour, T., Tondeur, S., Strom, S., Gabelle, A., Marty, S., Nadal, L., Pantesco, V., Reme, T., Hugnot, J. P., Gasca, S., Hovatta, O., Hamamah, S., Klein, B., & De Vos, J. (2007). A Meta-Analysis of Human Embryonic Stem Cells Transcriptome Integrated into a web-Based Expression Atlas. / Stem Cells, 25(4), 961鈥?73. CrossRef
16. Li, Y., & Ghosh, D. (2012). Assumption Weighting for Incorporating Heterogeneity into Meta-Analysis of Genomic Data. / Bioinformatics, 28(6), 807鈥?14. CrossRef
17. Wren, J. D., & Conway, T. (2006). Meta-Analysis of Published Transcriptional and Translational Fold Changes Reveals a Preference for low-Fold Inductions. / OMICS, 10(1), 15鈥?7. doi:10.1089/omi.2006.10.15 . CrossRef
18. Dennis, G., Jr., Sherman, B. T., Hosack, D. A., Yang, J., Gao, W., Lane, H. C., & Lempicki, R. A. (2003). DAVID: Database for Annotation, Visualization, and Integrated Discovery. / Genome Biology, 4(5), P3. CrossRef
19. Brown KR, Jurisica I (2007) Unequal evolutionary conservation of human protein interactions in interologous networks. Genome Biol 8 (5):R95. doi:gb-2007鈥?鈥?-r95
20. Smoot, M. E., Ono, K., Ruscheinski, J., Wang, P. L., & Ideker, T. (2011). Cytoscape 2.8: new Features for Data Integration and Network Visualization. / Bioinformatics, 27(3), 431鈥?32. CrossRef
21. Shan, Z. Y., Wu, Y. S., Li, X., Shen, X. H., Wang, Z. D., Liu, Z. H., Shen, J. L., & Lei, L. (2014). Continuous Passages Accelerate the Reprogramming of Mouse Induced Pluripotent Stem Cells. / Cellular Reprogramming, 16(1), 77鈥?3. doi:10.1089/cell.2013.0067 . CrossRef
22. Zheng, Z., Jia, J. L., Bou, G., Hu, L. L., Wang, Z. D., Shen, X. H., Shan, Z. Y., Shen, J. L., Liu, Z. H., & Lei, L. (2012). / The Journal of Biological Chemistry, 287(24), 19949鈥?9960. CrossRef
23. Tesarik, J., Rienzi, L., Ubaldi, F., Mendoza, C., & Greco, E. (2002). Use of a Modified Intracytoplasmic Sperm Injection Technique to Overcome Sperm-Borne and Oocyte-Borne Oocyte Activation Failures. / Fertility and Sterility, 78(3), 619鈥?24. CrossRef
24. Araki, Y., Yoshizawa, M., Abe, H., & Murase, Y. (2004). Use of Mouse Oocytes to Evaluate the Ability of Human Sperm to Activate Oocytes After Failure of Activation by Intracytoplasmic Sperm Injection. / Zygote, 12(2), 111鈥?16. CrossRef
25. Livak, K. J., & Schmittgen, T. D. (2001). Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2(鈭扗elta Delta C(T)) Method. / Methods, 25(4), 402鈥?08. doi:10.1006/meth.2001.1262 . CrossRef
26. Sridharan, R., Tchieu, J., Mason, M. J., Yachechko, R., Kuoy, E., Horvath, S., Zhou, Q., & Plath, K. (2009). Role of the Murine Reprogramming Factors in the Induction of Pluripotency. / Cell, 136(2), 364鈥?77. CrossRef
27. Maherali, N., Sridharan, R., Xie, W., Utikal, J., Eminli, S., Arnold, K., Stadtfeld, M., Yachechko, R., Tchieu, J., Jaenisch, R., Plath, K., & Hochedlinger, K. (2007). Directly Reprogrammed Fibroblasts Show Global Epigenetic Remodeling and Widespread Tissue Contribution. / Cell Stem Cell, 1(1), 55鈥?0. CrossRef
28. Li, H. Y., Chien, Y., Chen, Y. J., Chen, S. F., Chang, Y. L., Chiang, C. H., Jeng, S. Y., Chang, C. M., Wang, M. L., Chen, L. K., Hung, S. I., Huo, T. I., Lee, S. D., & Chiou, S. H. (2011). Reprogramming Induced Pluripotent Stem Cells in the Absence of c-Myc for Differentiation into Hepatocyte-Like Cells. / Biomaterials, 32(26), 5994鈥?005.
29. Laherty, C. D., Billin, A. N., Lavinsky, R. M., Yochum, G. S., Bush, A. C., Sun, J. M., Mullen, T. M., Davie, J. R., Rose, D. W., Glass, C. K., Rosenfeld, M. G., Ayer, D. E., & Eisenman, R. N. (1998). SAP30, a Component of the mSin3 Corepressor Complex Involved in N-CoR-Mediated Repression by Specific Transcription Factors. / Molecular Cell, 2(1), 33鈥?2. CrossRef
30. Fan, R., Bonde, S., Gao, P., Sotomayor, B., Chen, C., Mouw, T., Zavazava, N., & Tan, K. (2012). Dynamic HoxB4-Regulatory Network During Embryonic Stem Cell Differentiation to Hematopoietic Cells. / Blood, 119(19), e139鈥?47. CrossRef
31. Williams, K., Christensen, J., Pedersen, M. T., Johansen, J. V., Cloos, P. A., Rappsilber, J., & Helin, K. (2011). TET1 and Hydroxymethylcytosine in Transcription and DNA Methylation Fidelity. / Nature, 473(7347), 343鈥?48. CrossRef
32. Li, L., Zheng, P., & Dean, J. (2010). Maternal Control of Early Mouse Development. / Development, 137(6), 859鈥?70. CrossRef
33. Pierre, A., Gautier, M., Callebaut, I., Bontoux, M., Jeanpierre, E., Pontarotti, P., & Monget, P. (2007). Atypical Structure and Phylogenomic Evolution of the new Eutherian Oocyte and Embryo-Expressed KHDC1/DPPA5/ECAT1/OOEP Gene Family. / Genomics, 90(5), 583鈥?94. CrossRef
34. Schumacher, A., & Doerfler, W. (2004). Influence of in Vitro Manipulation on the Stability of Methylation Patterns in the Snurf/Snrpn-Imprinting Region in Mouse Embryonic Stem Cells. / Nucleic Acids Research, 32(4), 1566鈥?576. doi:10.1093/nar/gkh322 32/4/1566 . CrossRef
35. Dong, F., Song, Z., Zhang, J., Lu, Y., Song, C., Jiang, B., Zhang, B., Cong, P., Sun, H., Shi, F., & Liu, H. (2013). Global Transcriptional Analysis of Nuclear Reprogramming in the Transition from MEFs to iPSCs. / Genes to Cells, 18(1), 42鈥?5. CrossRef
36. Comes, S., Gagliardi, M., Laprano, N., Fico, A., Cimmino, A., Palamidessi, A., De Cesare, D., De Falco, S., Angelini, C., Scita, G., Patriarca, E. J., Matarazzo, M. R., & Minchiotti, G. (2013). L-Proline Induces a Mesenchymal-like Invasive Program in Embryonic Stem Cells by Remodeling H3K9 and H3K36 Methylation. / Stem Cell Reports, 1(4), 307鈥?21. CrossRef
37. Huggins, C. J., Malik, R., Lee, S., Salotti, J., Thomas, S., Martin, N., Quinones, O. A., Alvord, W. G., Olanich, M. E., Keller, J. R., & Johnson, P. F. (2013). C/EBPgamma Suppresses Senescence and Inflammatory Gene Expression by Heterodimerizing With C/EBPbeta. / Molecular and Cellular Biology, 33(16), 3242鈥?258. CrossRef
38. Ho, R., Papp, B., Hoffman, J. A., Merrill, B. J., & Plath, K. (2013). Stage-Specific Regulation of Reprogramming to Induced Pluripotent Stem Cells by Wnt Signaling and T Cell Factor Proteins. / Cell Reports, 3(6), 2113鈥?126. CrossRef
39. Martinez-Fernandez, A., Li, X., Hartjes, K. A., Terzic, A., & Nelson, T. J. (2013). Natural Cardiogenesis-Based Template Predicts Cardiogenic Potential of Induced Pluripotent Stem Cell Lines. / Circulation. Cardiovascular Genetics, 6(5), 462鈥?71. CrossRef
40. Teske, B. F., Fusakio, M. E., Zhou, D., Shan, J., McClintick, J. N., Kilberg, M. S., & Wek, R. C. (2013). CHOP Induces Activating Transcription Factor 5 (ATF5) to Trigger Apoptosis in Response to Perturbations in Protein Homeostasis. / Molecular Biology of the Cell, 24(15), 2477鈥?490. CrossRef
41. Oda, M., Kumaki, Y., Shigeta, M., Jakt, L. M., Matsuoka, C., Yamagiwa, A., Niwa, H., & Okano, M. (2013). DNA Methylation Restricts Lineage-Specific Functions of Transcription Factor Gata4 During Embryonic Stem Cell Differentiation. / PLoS Genetics, 9(6), e1003574. CrossRef
42. Yeung, F., Ramirez, C. M., Mateos-Gomez, P. A., Pinzaru, A., Ceccarini, G., Kabir, S., Fernandez-Hernando, C., & Sfeir, A. (2013). Nontelomeric Role for Rap1 in Regulating Metabolism and Protecting Against Obesity. / Cell Reports, 3(6), 1847鈥?856. CrossRef
43. Li, W., Shuai, L., Wan, H., Dong, M., Wang, M., Sang, L., Feng, C., Luo, G. Z., Li, T., Li, X., Wang, L., Zheng, Q. Y., Sheng, C., Wu, H. J., Liu, Z., Liu, L., Wang, X. J., Zhao, X. Y., & Zhou, Q. (2012). Androgenetic Haploid Embryonic Stem Cells Produce Live Transgenic Mice. / Nature, 490(7420), 407鈥?11. CrossRef
44. Jiang, J., Ding, G., Lin, J., Zhang, M., Shi, L., Lv, W., Yang, H., Xiao, H., Pei, G., Li, Y., Wu, J., & Li, J. (2011). Different Developmental Potential of Pluripotent Stem Cells Generated by Different Reprogramming Strategies. / Journal of Molecular Cell Biology, 3(3), 197鈥?99. CrossRef
45. Matsui, T., Takano, M., Yoshida, K., Ono, S., Fujisaki, C., Matsuzaki, Y., Toyama, Y., Nakamura, M., Okano, H., & Akamatsu, W. (2012). Neural Stem Cells Directly Differentiated from Partially Reprogrammed Fibroblasts Rapidly Acquire Gliogenic Competency. / Stem Cells, 30(6), 1109鈥?119. CrossRef
46. Kuckenberg, P., Peitz, M., Kubaczka, C., Becker, A., Egert, A., Wardelmann, E., Zimmer, A., Brustle, O., & Schorle, H. (2011). Lineage Conversion of Murine Extraembryonic Trophoblast Stem Cells to Pluripotent Stem Cells. / Molecular and Cellular Biology, 31(8), 1748鈥?756. CrossRef
47. Nakagawa, S., Ip, J. Y., Shioi, G., Tripathi, V., Zong, X., Hirose, T., & Prasanth, K. V. (2012). Malat1 is not an Essential Component of Nuclear Speckles in Mice. / RNA, 18(8), 1487鈥?499. CrossRef
48. Chen, J., Liu, J., Han, Q., Qin, D., Xu, J., Chen, Y., Yang, J., Song, H., Yang, D., Peng, M., He, W., Li, R., Wang, H., Gan, Y., Ding, K., Zeng, L., Lai, L., Esteban, M. A., & Pei, D. (2010). Towards an Optimized Culture Medium for the Generation of Mouse Induced Pluripotent Stem Cells. / The Journal of Biological Chemistry, 285(40), 31066鈥?1072. CrossRef
49. Yang, H., Shi, L., Wang, B. A., Liang, D., Zhong, C., Liu, W., Nie, Y., Liu, J., Zhao, J., Gao, X., Li, D., Xu, G. L., & Li, J. (2012). Generation of Genetically Modified Mice by Oocyte Injection of Androgenetic Haploid Embryonic Stem Cells. / Cell, 149(3), 605鈥?17. CrossRef
50. Zhu, B. M., Kang, K., Yu, J. H., Chen, W., Smith, H. E., Lee, D., Sun, H. W., Wei, L., & Hennighausen, L. (2012). Genome-Wide Analyses Reveal the Extent of Opportunistic STAT5 Binding That Does not Yield Transcriptional Activation of Neighboring Genes. / Nucleic Acids Research, 40(10), 4461鈥?472. CrossRef
51. Faustino, R. S., Chiriac, A., Niederlander, N. J., Nelson, T. J., Behfar, A., Mishra, P. K., Macura, S., Michalak, M., Terzic, A., & Perez-Terzic, C. (2010). Decoded Calreticulin-Deficient Embryonic Stem Cell Transcriptome Resolves Latent Cardiophenotype. / Stem Cells, 28(7), 1281鈥?291.
52. Liu, J., Johnson, K., Li, J., Piamonte, V., Steffy, B. M., Hsieh, M. H., Ng, N., Zhang, J., Walker, J. R., Ding, S., Muneoka, K., Wu, X., Glynne, R., & Schultz, P. G. (2011). Regenerative Phenotype in Mice With a Point Mutation in Transforming Growth Factor Beta Type I Receptor (TGFBR1). / Proceedings of the National Academy of Sciences of the United States of America, 108(35), 14560鈥?4565.
53. Kleger, A., Seufferlein, T., Malan, D., Tischendorf, M., Storch, A., Wolheim, A., Latz, S., Protze, S., Porzner, M., Proepper, C., Brunner, C., Katz, S. F., Varma Pusapati, G., Bullinger, L., Franz, W. M., Koehntop, R., Giehl, K., Spyrantis, A., Wittekindt, O., Lin, Q., Zenke, M., Fleischmann, B. K., Wartenberg, M., Wobus, A. M., Boeckers, T. M., & Liebau, S. (2010). Modulation of Calcium-Activated Potassium Channels Induces Cardiogenesis of Pluripotent Stem Cells and Enrichment of Pacemaker-Like Cells. / Circulation, 122(18), 1823鈥?836. CrossRef
54. Takii, R., Inouye, S., Fujimoto, M., Nakamura, T., Shinkawa, T., Prakasam, R., Tan, K., Hayashida, N., Ichikawa, H., Hai, T., & Nakai, A. (2010). Heat Shock Transcription Factor 1 Inhibits Expression of IL-6 Through Activating Transcription Factor 3. / The Journal of Immunology, 184(2), 1041鈥?048. CrossRef
55. Ko, K., Tapia, N., Wu, G., Kim, J. B., Bravo, M. J., Sasse, P., Glaser, T., Ruau, D., Han, D. W., Greber, B., Hausdorfer, K., Sebastiano, V., Stehling, M., Fleischmann, B. K., Brustle, O., Zenke, M., & Scholer, H. R. (2009). Induction of Pluripotency in Adult Unipotent Germline Stem Cells. / Cell Stem Cell, 5(1), 87鈥?6. CrossRef
56. Liu, L., Luo, G. Z., Yang, W., Zhao, X., Zheng, Q., Lv, Z., Li, W., Wu, H. J., Wang, L., Wang, X. J., & Zhou, Q. (2010). Activation of the Imprinted Dlk1-Dio3 Region Correlates With Pluripotency Levels of Mouse Stem Cells. / The Journal of Biological Chemistry, 285(25), 19483鈥?9490. CrossRef
57. Zhao, X. Y., Li, W., Lv, Z., Liu, L., Tong, M., Hai, T., Hao, J., Guo, C. L., Ma, Q. W., Wang, L., Zeng, F., & Zhou, Q. (2009). iPS Cells Produce Viable Mice Through Tetraploid Complementation. / Nature, 461(7260), 86鈥?0. CrossRef
58. Labalette, C., Nouet, Y., Sobczak-Thepot, J., Armengol, C., Levillayer, F., Gendron, M. C., Renard, C. A., Regnault, B., Chen, J., Buendia, M. A., & Wei, Y. (2008). The LIM-Only Protein FHL2 Regulates Cyclin D1 Expression and Cell Proliferation. / The Journal of Biological Chemistry, 283(22), 15201鈥?5208. CrossRef
59. Labalette, C., Nouet, Y., Levillayer, F., Armengol, C., Renard, C. A., Soubigou, G., Xia, T., Buendia, M. A., & Wei, Y. (2008). The LIM-Only Protein FHL2 Mediates ras-Induced Transformation Through Cyclin D1 and p53 Pathways. / PloS One, 3(11), e3761. CrossRef
60. Kim, J. B., Zaehres, H., Wu, G., Gentile, L., Ko, K., Sebastiano, V., Arauzo-Bravo, M. J., Ruau, D., Han, D. W., Zenke, M., & Scholer, H. R. (2008). Pluripotent Stem Cells Induced from Adult Neural Stem Cells by Reprogramming With two Factors. / Nature, 454(7204), 646鈥?50. CrossRef
61. Ralston, A., Cox, B. J., Nishioka, N., Sasaki, H., Chea, E., Rugg-Gunn, P., Guo, G., Robson, P., Draper, J. S., & Rossant, J. (2010). Gata3 Regulates Trophoblast Development Downstream of Tead4 and in Parallel to Cdx2. / Development, 137(3), 395鈥?03. CrossRef
62. Rampon, C., Bouillot, S., Climescu-Haulica, A., Prandini, M. H., Cand, F., Vandenbrouck, Y., & Huber, P. (2008). Protocadherin 12 Deficiency Alters Morphogenesis and Transcriptional Profile of the Placenta. / Physiological Genomics, 34(2), 193鈥?04. CrossRef
63. Faustino, R. S., Behfar, A., Perez-Terzic, C., & Terzic, A. (2008). Genomic Chart Guiding Embryonic Stem Cell Cardiopoiesis. / Genome Biology, 9(1), R6. CrossRef
64. Hui, L., Bakiri, L., Mairhorfer, A., Schweifer, N., Haslinger, C., Kenner, L., Komnenovic, V., Scheuch, H., Beug, H., & Wagner, E. F. (2007). p38alpha Suppresses Normal and Cancer Cell Proliferation by Antagonizing the JNK-c-Jun Pathway. / Nature Genetics, 39(6), 741鈥?49. CrossRef
65. Klose, R. J., Yan, Q., Tothova, Z., Yamane, K., Erdjument-Bromage, H., Tempst, P., Gilliland, D. G., Zhang, Y., & Kaelin, W. G., Jr. (2007). The Retinoblastoma Binding Protein RBP2 is an H3K4 Demethylase. / Cell, 128(5), 889鈥?00. CrossRef
66. Koziczak-Holbro, M., Joyce, C., Gluck, A., Kinzel, B., Muller, M., Tschopp, C., Mathison, J. C., Davis, C. N., & Gram, H. (2007). IRAK-4 Kinase Activity is Required for Interleukin-1 (IL-1) Receptor and Toll-Like Receptor 7-Mediated Signaling and Gene Expression. / The Journal of Biological Chemistry, 282(18), 13552鈥?3560. CrossRef - 作者单位：Lian Duan (1)
Zhendong Wang (1)
Jingling Shen (1) (2)
Zhiyan Shan (1) (2)
Xinghui Shen (1) (2)
Yanshuang Wu (1) (2)
Ruizhen Sun (1) (2)
Tong Li (1)
Rui Yuan (1)
Qiaoshi Zhao (1)
Guangyu Bai (1)
Yanli Gu (1)
Lianhong Jin (1) (2)
Lei Lei (1) (2)
1. Department of Histology and Embryology, Harbin Medical University, 194 Xuefu Road, Harbin, China
2. Embryo and Stem Cell Engineering Laboratory, Harbin Medical University, Harbin, China - ISSN：1558-6804
文摘
The most effective reprogramming methods, somatic cell nuclear transfer (SCNT) and induced pluripotent stem cells (iPSCs), are widely used in biological research and regenerative medicine, yet the mechanism that reprograms somatic cells to totipotency remains unclear and thus reprogramming efficiency is still low. Microarray technology has been employed in analyzing the transcriptomes changes during iPS reprogramming. Unfortunately, it is difficult to obtain enough DNA from SCNT reconstructed embryos to take advantage of this technology. In this study, we aimed to identify critical genes from the transcriptional profile for iPS reprogramming and compared expression levels of these genes in SCNT reprogramming. By integrating gene expression information from microarray databases and published studies comparing somatic cells with either miPSCs or mouse embryonic stem cells (ESCs), we obtained two lists of co-upregulated genes. The gene ontology (GO) enriched analysis of these two lists demonstrated that the reprogramming process is associated with numerous biological processes. Specifically, we selected 32 genes related to heterochromatin, embryonic development, and cell cycle from our co-upregulated gene datasets and examined the gene expression level in iPSCs and SCNT embryos by qPCR. The results revealed that some reprogramming related genes in iPSCs were also expressed in SCNT reprogramming. We established the network of gene interactions that occur with genes differentially expressed in iPS and SCNT reprogramming and then performed GO analysis on the genes in the network. The network genes function in chromatin organization, heterochromatin, transcriptional regulation, and cell cycle. Further researches to improve reprogramming efficiency, especially in SCNT, will focus on functional studies of these selected genes.