BEFS-PPARγ2 cells incubated with trans-11 C18:1 exhibit more beneficial fatty acid synthesis

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• 作者：Tao Wang ; HongGu Lee ; LiFang Wu ; GuiXin Qin ; YuJie Lou…
• 关键词：BEFS ; PPARγ2 cell ; trans ; 11 C18 ; 1 ; cis ; 9 ; trans ; 11 CLA
• 刊名：Food Science and Biotechnology
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：24
• 期：5
• 页码：1893-1896
• 全文大小：338 KB
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• 作者单位：Tao Wang (1)
  HongGu Lee (2)
  LiFang Wu (1)
  GuiXin Qin (1)
  YuJie Lou (1)
  ZeWei Sun (1)
  Wei Guo (3)
  Jian Yang (1)
  
  1. College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China
  2. Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University, Seoul, 143-701, Korea
  3. Animal Husbandry Station of Jilin Province, Changchun, 130118, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Nutrition
  Food Science
  
• 出版者：The Korean Society of Food Science and Technology in co-publication with Springer
• ISSN：2092-6456

文摘

Responses of the PPARγ2 (an adipogenic lineage determinant) over-expressed immortalized bovine embryonic fibroblast cell line (BEFS-PPARγ2 cells) to trans-11 C18:1 (TVA) incubation were evaluated. BEFS-PPARγ2 cells were well differentiated. Stearoyl-CoA desaturase 1, an endoplasmic reticulum enzyme that can catalyze conversion of TVA into cis-9, trans-11 CLA, exhibited a significantly (p<0.05) higher mRNA expression after 4 h, compared with controls. Enzyme activity peaked, and was significantly (p<0.05) higher after 6 h, compared with controls. With extension of the incubation time, TVA absorption into cells was significantly (p<0.05) higher with significantly (p<0.05) more conversion into cis-9, trans-11 CLA, compared with shorter incubation times. TVA incubated cells exhibited significantly (p<0.05) higher levels of arachidonic, eicosapentaenoic, and docosahexaenoic acids than controls. BEFS-PPARγ2 cells can be used as a model system and TVA incubation is a good approach for research into cis-9, trans-11 CLA-fortified beef production. Keywords BEFS-PPARγ2 cell trans-11 C18:1 cis-9 trans-11 CLA