Integrated omics analyses reveal the details of metabolic adaptation of Clostridium thermocellum to lignocellulose-derived growth inhibitors released during the deconstruction of switchgrass

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• 作者：Suresh Poudel ; Richard J. Giannone ; Miguel Rodriguez Jr.…
• 关键词：Clostridium thermocellum ; Switchgrass ; Lignocellulosic ; Biofuel ; Ethanol ; Mass spectrometry ; Proteomics ; Metabolomics ; Transcriptomics ; Cellulosome
• 刊名：Biotechnology for Biofuels
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：10
• 期：1
• 全文大小：1787KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Biotechnology; Plant Breeding/Biotechnology; Environmental Engineering/Biotechnology; Renewable and Green Energy; Renewable and Green Energy; Microbiology;
• 出版者：BioMed Central
• ISSN：1754-6834
• 卷排序：10

文摘

BackgroundClostridium thermocellum is capable of solubilizing and converting lignocellulosic biomass into ethanol. Although much of the work-to-date has centered on characterizing this microbe’s growth on model cellulosic substrates, such as cellobiose, Avicel, or filter paper, it is vitally important to understand its metabolism on more complex, lignocellulosic substrates to identify relevant industrial bottlenecks that could undermine efficient biofuel production. To this end, we have examined a time course progression of C. thermocellum grown on switchgrass to assess the metabolic and protein changes that occur during the conversion of plant biomass to ethanol.