Determination of Gastrodin and Ligustrazine Hydrochloride in Plasma and Brain Dialysate by LC¨CTandem MS

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• 作者：Yun-Feng Lv ; Xin Hu ; Wei-Ming Cheng ; Ying-Lan Nie and Kai-Shun Bi
• 关键词：Column liquid chromatography– ; tandem mass spectrometry ; Microdialysis ; Gastrodin and ligustrazine hydrochloride ; Plasma and brain dialysate samples
• 刊名：Chromatographia
• 出版年：2008
• 出版时间：July, 2008
• 年：2008
• 卷：68
• 期：1-2
• 页码：105-110
• 全文大小：368.5 KB

文摘

A gradient liquid chromatography-tandem mass spectrometry method has been developed and validated for the determination of gastrodin and ligustrazine hydrochloride in rat plasma and brain dialysates. Zolpidem was used as internal standard. For plasma samples, solid-phase extraction was used and the brain dialysates were collected from freely moving rats using brain microdialysis. Both were followed by HPLC separation and positive electrospray ionization tandem mass spectrometry detection (ESI–MS–MS). Chromatographic separation was achieved on a Symmetry RP-18 column using gradient elution with methanol and water containing 0.5 % formic acid and 2 mM ammonium formate. Selected reaction monitoring (SRM) mode was used for quantitation. Good linearities were obtained in the range of 0.05–100 and 0.01–50 μg mL−1 for gastrodin and ligustrazine hydrochloride in rat plasma, and 0.05–1,000 ng mL−1 for both in dialysate. The lower limit of quantitation was 0.01 ng mL−1 for gastrodin and 0.05 ng mL−1 for ligustrazine. The method is precise and reliable and can be applied to pharmacokinetic studies.