The gene PmWFJ is a new member of the complex Pm2 locus conferring unique powdery mildew resistance in wheat breeding line Wanfengjian 34

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• 作者：Pengtao Ma ; Hongxng Xu ; Hongxia Zhang ; Lihui Li ; Yunfeng Xu…
• 关键词：Breeding line ; Powdery mildew ; Multi ; allelic Pm2 locus ; MAS ; Triticum aestivum
• 刊名：Molecular Breeding
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：35
• 期：11
• 全文大小：2,100 KB
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• 作者单位：Pengtao Ma (1)
  Hongxng Xu (1)
  Hongxia Zhang (1)
  Lihui Li (2)
  Yunfeng Xu (1)
  Xiaotian Zhang (1)
  Diaoguo An (1)
  
  1. Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, 050021, China
  2. The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  
• 出版者：Springer Netherlands
• ISSN：1572-9788

文摘

Molecular identification of wheat powdery mildew resistance (Pm) genes in breeding lines or cultivars is important for resistance breeding. Wanfengjian 34, a Chinese wheat breeding line with desirable agricultural performance in China, showed high level of resistance to powdery mildew at both seedling and adult stages. Genetic analysis demonstrated that the resistance to different Blumeria graminis f. sp. tritici isolates in Wanfengjian 34 was controlled by a single dominant Pm gene, temporarily designated PmWFJ. PmWFJ was mapped to chromosome arm 5DS and flanked by the simple sequence repeat marker Cfd81 and sequence characterized amplified region marker SCAR112 with genetic distance of 0.1 and 1.0 cM, respectively. PmWFJ shared the same locus with the documented Pm genes on the multi-allelic Pm2 locus by allelism tests between PmWFJ and Pm2 alleles, but showed unique powdery mildew reaction pattern compared with these documented Pm genes. Therefore, PmWFJ appears to be a new allele at the documented Pm2 locus. Four closely linked markers of PmWFJ were validated the applicability in 131 cultivar backgrounds for marker-assisted selection.