Cloning and expression of anthocyanin biosynthetic genes in red and white pomegranate

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• 作者：Xueqing Zhao ; Zhaohe Yuan ; Lijuan Feng ; Yanming Fang
• 关键词：Anthocyanin ; Gene cloning ; Gene expression ; Pomegranate
• 刊名：Journal of Plant Research
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：128
• 期：4
• 页码：687-696
• 全文大小：799 KB
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• 作者单位：Xueqing Zhao (1) (2)
  Zhaohe Yuan (1) (3)
  Lijuan Feng (1) (2)
  Yanming Fang (1) (2)
  
  1. Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 159 Longpan Rd., Nanjing, 210037, China
  2. College of Biology and the Environment, Nanjing Forestry University, 159 Longpan Rd., Nanjing, 210037, China
  3. College of Forestry, Nanjing Forestry University, 159 Longpan Rd., Nanjing, 210037, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Plant Ecology
  Plant Physiology
  Plant Biochemistry
  
• 出版者：Springer Japan
• ISSN：1618-0860

文摘

Exterior fruit color is an important trait for the evaluation of pomegranate fruit quality, but the molecular mechanism underlying the variation in color between red- and white-fruited pomegranate is poorly understood. In this study, full-length cDNA clones encoding enzymes involved in anthocyanin biosynthesis—such as chalcone synthase, chalcone isomerase, flavanone 3-hydoxylase, dihydroflavonol 4-reductase, anthocyanidin synthase (ANS), UDP-glucose-flavonoid 3-O-glucosyltransferase, and the R2R3 MYB transcription factor PgMYB—were isolated from fruit peels. In addition, transcript levels of anthocyanin biosynthetic genes were quantitatively measured by real-time PCR in red and white fruits. In both cultivars, two expression peaks for structural genes were detected during fruit development, whereas only one peak was observed—during early development—for PgMYB. While PgMYB is important for flavonoid biosynthesis, other transcription factors appear to also be necessary for the regulation of anthocyanin biosynthesis. No anthocyanins were detected in the white cultivar. Peels of white fruits contained transcripts of all identified genes except for PgANS, suggesting that the lack of PgANS expression may be the main factor responsible for the absence of anthocyanins in white pomegranate. PgANS may be the key gene involved in anthocyanin biosynthesis in pomegranate fruit.