Multi-breed genome-wide association study reveals heterogeneous loci associated with loin eye area in pigs
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- 作者：Yuna He ; Junwu Ma ; Feng Zhang ; Lijuan Hou ; Hao Chen…
- 关键词：GWAS ; Heterogeneous ; Loin eye area ; Pig
- 刊名：Journal of Applied Genetics
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：57
- 期：4
- 页码：511-518
- 全文大小：1,091 KB
- 刊物主题：Life Sciences, general; Animal Genetics and Genomics; Human Genetics; Microbial Genetics and Genomics; Plant Genetics & Genomics;
- 出版者：Springer Berlin Heidelberg
- ISSN：2190-3883
- 卷排序：57
文摘
Numerous quantitative trait loci (QTL) for loin eye area had been identified by linkage mapping studies, but the lack of their precise position hinders their application in the pig breeding industry. To map QTL for loin eye area to a precise genomic region, we conducted a genome-wide association study (GWAS) using Illumina 60 K PorcineSNP60 Beadchip in four swine populations: 819 F2 pigs, 273 Laiwu pigs, 434 Sutai pigs, and 326 Erhualian pigs. In total, 26 single nucleotide polymorphisms (SNPs) deposited on seven chromosomes associated with loin eye area were identified, 11 of which surpassed the genome-wide significant threshold; of the 11 SNPs, seven located on SSC2 in F2 pigs and four located on SSC12 and SSC18 in Laiwu pigs. Of note, all of the identified QTL were breed specific and no common QTL was identified across the four populations in our study. These findings not only confirmed a previous QTL on SSC2 harboring the candidate gene insulin-like growth factor 2 (IGF2), but also identified some novel candidate genes, far upstream element binding protein 3 (FUBP3), myosin heavy chain (MYH) family, leucine-rich repeats and guanylate kinase domain containing (LRGUK). Our study will contribute to the further identification of the causal mutation underlying these QTL and improve our knowledge of the complex genetic architecture for loin eye area in pigs.