H2O2 Induces Myocardial Hypertrophy in H9c2 Cells: A Potential Role of Ube3a

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• 作者：Rui Song ; Jie Zhang ; Lijuan Zhang ; Guanghua Wang ; Da Wo…
• 关键词：H2O2 ; Ube3a ; Myocardial hypertrophy ; Ubiquitin
• 刊名：Cardiovascular Toxicology
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：15
• 期：1
• 页码：23-28
• 全文大小：619 KB
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• 作者单位：Rui Song (1) (2)
  Jie Zhang (1) (2)
  Lijuan Zhang (1) (2)
  Guanghua Wang (1) (2)
  Da Wo (2)
  Jian Feng (1) (2)
  Xucheng Li (1) (2)
  Jue Li (1) (2)
  
  1. Department of Prevention, Tongji University School of Medicine, Shanghai, China
  2. Key Laboratory of Arrhythmias, Ministry of Education, Tongji University School of Medicine, Shanghai, China
  
• 刊物主题：Pharmacology/Toxicology; Cardiology;
• 出版者：Springer US
• ISSN：1559-0259

文摘

Myocardial hypertrophy that often leads to eventual heart failure is a leading cause of mortality worldwide. While both apoptosis and cell proliferation have been reported to play an important part in heart failure, its exact triggering mechanism is still unclear. Reports have shown that low concentrations of H2O2 (10-0?μM) can induce myocardial hypertrophy without affecting survival. The ubiquitin ligase Ube3a has been reported to have a close affiliation with Angelman syndrome; but many ubiquitin ligases have been reported in a variety of cardiovascular conditions including myocardial hypertrophy. However, the relationship between Ube3a and myocardial hypertrophy has never been reported in literature. The rat cardiac myoblast cell line H9c2 and primary neonatal cardiomyocytes showed similar hypertrophic responses in vitro. In this report, we utilized H2O2 treatment on H9c2 cells to induce myocardial hypertrophy and determined the relationship between Ube3a and myocardial hypertrophy. Our results showed that 10-0?μM H2O2 can induce myocardial hypertrophy without affecting cell viability and inducing cell apoptosis, while the corresponding transcription and translation levels of Ube3a are significantly increased during the process. Therefore, these findings underline that Ube3a may play an important role in myocardial hypertrophy.