Cytocompatible 3D chitosan/hydroxyapatite composites endowed with antibacterial properties: toward a self-sterilized bone tissue engineering scaffold

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• 作者：Yongjun Qiao ; Zhongjun Zhai ; Limei Chen ; Hong Liu
• 关键词：Silver phosphate ; Ion substitution ; Chitosan/nano ; hydroxyapatite bone scaffold ; Bacteriostasis ; /li> ; ;
• 刊名：Chinese Science Bulletin
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：60
• 期：13
• 页码：1193-1202
• 全文大小：1,663 KB
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• 作者单位：Yongjun Qiao (1) (2)
  Zhongjun Zhai (3)
  Limei Chen (2)
  Hong Liu (2) (3)
  
  1. Industry Group of Shandong University, Jinan, 250100, China
  2. State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, China
  3. Innowit Co., Ltd, Jinan, 250101, China
  
• 刊物主题：Science, general; Life Sciences, general; Physics, general; Chemistry/Food Science, general; Earth Sciences, general; Engineering, general;
• 出版者：Springer Berlin Heidelberg
• ISSN：1861-9541

文摘

Bone tissue scaffolds based on bioactive polymer–hydroxyapatite composites have caused infections that seriously limit their extended application. In this study, we proposed a practical ion substitution method to synthesize in situ silver phosphate on the surface of a two-level, three-dimensional chitosan/nano-hydroxyapatite scaffold. A release test of silver ions in a phosphate buffered saline (PBS) solution was performed to demonstrate that silver ions were released continuously from the silver phosphate during the initial 6days of the study. The antibacterial property and cytocompatibility of the scaffolds treated with different concentrations of silver nitrate solution were assessed by in vitro assays with Escherichia coli and MC3T3-E1, respectively. The ability of the silver-containing scaffolds to induce bacteriostasis was confirmed by the inhibition zone (15mm) and high bactericidal rate (>99%). Cell proliferation, morphology and the alkaline phosphatase activity of MC3T3-E1 cultured on the scaffold with low silver phosphate contents were comparable with those cultured on control samples.