Hepatic Metallothionein and Glutathione-S-Transferase Responses in Two Populations of Rice Frogs, Fejervarya limnocharis, Naturally Exposed to Different Environmental Cadmium Levels

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• 作者：Mohd Sham Othman (1) (2) (3) (4)
  Wichase Khonsue (3) (4)
  Jirarach Kitana (3) (4)
  Kumthorn Thirakhupt (3) (4)
  Mark Robson (3) (5) (6)
  Marija Borjan (5) (6)
  Noppadon Kitana (3) (4)
  
• 关键词：Cd ; Fejervarya limnocharis ; Metallothionein ; GST ; Biomarker
• 刊名：Bulletin of Environmental Contamination and Toxicology
• 出版年：2012
• 出版时间：August 2012
• 年：2012
• 卷：89
• 期：2
• 页码：225-228
• 全文大小：206KB
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• 作者单位：Mohd Sham Othman (1) (2) (3) (4)
  Wichase Khonsue (3) (4)
  Jirarach Kitana (3) (4)
  Kumthorn Thirakhupt (3) (4)
  Mark Robson (3) (5) (6)
  Marija Borjan (5) (6)
  Noppadon Kitana (3) (4)
  
  1. Environmental Health & Industrial Safety Program, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia
  2. International Postgraduate Programs in Environmental Management, Graduate School, Chulalongkorn University, Bangkok, Thailand
  3. National Center of Excellence for Environmental and Hazardous Waste Management (NCE-EHWM), Chulalongkorn University, Bangkok, Thailand
  4. Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand
  5. School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ, USA
  6. Thai Fogarty International Training and Research in Environmental and Occupational Health Center, Chulalongkorn University, Bangkok, Thailand
  
• ISSN：1432-0800

文摘

Glutathione-S-Transferase (GST) and metallothionein are important biomarker endpoints in studying the effect of Cd exposure. The purpose of this research was to study the correlation between hepatic GST and metallothionein with hepatic Cd in wild Fejervarya limnocharis exposed to environmental Cd. Results showed that frogs from contaminated sites had significantly higher hepatic metallothionein (3.58?mg/kg wet weight) and GST activity (0.259?μmol/min/mg total protein) than those from the reference site (2.36?mg/kg wet weight and 0.157?μmol/min/mg total protein respectively). There was a significantly positive correlation between hepatic Cd and GST activity (r?=?0.802, p?=?0.009) but not between hepatic Cd and metallothionein (r?=?0.548, p?=?0.139). The results concluded that while frogs from the contaminated site had higher GST and metallothionein, only GST showed significant positive correlation with hepatic Cd levels, indicating that hepatic GST activity may be used as a biomarker endpoint.