A serological and bacteriological survey of brucellosis in wild boar (Sus scrofa) in Belgium

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• 作者：Fabien Grégoire (1)
  Bénédicte Mousset (1)
  David Hanrez (1)
  Charles Michaux (2)
  Karl Walravens (3)
  Annick Linden (1)
  
• 刊名：BMC Veterinary Research
• 出版年：2012
• 出版时间：December 2012
• 年：2012
• 卷：8
• 期：1
• 全文大小：375KB
• 参考文献：1. Godfroid J, K?sbohrer A: g class="a-plus-plus">Brucellosis in the European Union and Norway at the turn of the twenty-first century.g> / Vet Microbiol 2002, g class="a-plus-plus">90:g>135-45. g/10.1016/S0378-1135(02)00217-1">CrossRef
  2. Godfroid J, Michel P, Uytterhaegen L, De Smedt C, Rasseneur F, Boelaert F, Saegerman C, Patigny X: g class="a-plus-plus">Brucellose enzootique à Brucella suis biotype 2 chez le sanglier (sus scrofa) en Belgique.g> / Ann Med Vet 1994, g class="a-plus-plus">138:g>263-68.
  3. Hars J, Thiebaud M, Cau C, Rossi S, Baudet E, Boué F, Garin-Bastuji B: g class="a-plus-plus">La brucellose du sanglier et du lièvre due à Brucella suis 2 en France.g> / Faune sauvage 2004, g class="a-plus-plus">261:g>18-3.
  4. Leuenberger R, Boujon P, Thur B, Miserez R, Garin-Bastuji B, Rufenacht J, Stark KD: g class="a-plus-plus">Prevalence of classical swine fever, Aujeszky's disease and brucellosis in a population of wild boar in Switzerland.g> / Vet Rec 2007,g class="a-plus-plus">160g>(11)g class="a-plus-plus">:g>362-68. g/10.1136/vr.160.11.362">CrossRef
  5. Wu N, Abril C, Hinic V, Brodard I, Thur B, Fattebert J, Hussy D, Ryser-Degiorgis MP: g class="a-plus-plus">Free-ranging wild boar: a disease threat to domestic pigs in Switzerland?g> / J Wildl Dis 2011,g class="a-plus-plus">47g>(4)g class="a-plus-plus">:g>868-79.
  6. Melzer F, Lohse R, Nieper H, Liebert M, Sachse K: g class="a-plus-plus">A serological study on brucellosis in wild boars in Germany.g> / Eur J Wildl Res 2007, g class="a-plus-plus">53:g>153-57. g/10.1007/s10344-006-0072-0">CrossRef
  7. Closa-Sebastia F, Casas-Diaz E, Cuenca R, Lavin S, Mentaberre G, Marco I: g class="a-plus-plus">Brucella species antibodies and isolation in wild boar in north-east Spain.g> / Vet Rec 2010,g class="a-plus-plus">167g>(21)g class="a-plus-plus">:g>826-28. g/10.1136/vr.c5927">CrossRef
  8. Munoz PM, Boadella M, Arnal M, de Miguel MJ, Revilla M, Martinez D, Vicente J, Acevedo P, Oleaga A, Ruiz-Fons F, / et al.: g class="a-plus-plus">Spatial distribution and risk factors of Brucellosis in Iberian wild ungulates.g> / BMC Infect Dis 2010, g class="a-plus-plus">10:g>46. g/10.1186/1471-2334-10-46">CrossRef
  9. Cvetnic Z, Mitak M, Ocepek M, Lojkic M, Terzic S, Jemersic L, Humski A, Habrun B, Sostaric B, Brstilo M, Krt B, Garin-Bastuji B: g class="a-plus-plus">Wild boars (Sus scrofa) as reservoirs of Brucella suis biovar 2 in Croatia.g> / Acta Vet Hung 2003,g class="a-plus-plus">51g>(4)g class="a-plus-plus">:g>465-73. g/10.1556/AVet.51.2003.4.4">CrossRef
  10. Statistiques sur le grand gibier en Région wallonne: / Edited by Service public de Wallonie, Direction générale opérationnelle de l'Agriculture, Département de la Nature et des Forêts, Filière cynégétique. Update March. 2011.
  11. Gortazar C, Ferroglio E, Hofle U, Fr?lich K, Vicente J: g class="a-plus-plus">Diseases shared between wildlife and livestock: a European perspective.g> / Eur J Wildlife Res 2007,g class="a-plus-plus">53g>(4)g class="a-plus-plus">:g>241-56. g/10.1007/s10344-007-0098-y">CrossRef
  12. World Organisation for Animal Health (OIE): / Chapter 2.8.5 Porcine Brucellosis. In Manual for Diagnosis Tests and Vaccines for Terrestrial Animals. 2011. access online. g/Health_standards/tahm/2.08.05_PORCINE_BRUC.pdf" class="a-plus-plus">http://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/2.08.05_PORCINE_BRUC.pdf
  13. Godfroid J: g class="a-plus-plus">Brucellosis in wildlife.g> / Rev sci tech Off int Epiz 2002,g class="a-plus-plus">21g>(2)g class="a-plus-plus">:g>277-86.
  14. Cvetnic Z, Spicic S, Toncic J, Majnaric D, Benic M, Albert D, Thiebaud M, Garin-Bastuji B: g class="a-plus-plus">Brucella suis infection in domestic pigs and wild boar in Croatia.g> / Rev Sci Tech 2009,g class="a-plus-plus">28g>(3)g class="a-plus-plus">:g>1057-067.
  15. INVS: / Etude sur les brucelloses humaines en France métropolitaine, 2002-004. Saint-Maurice, France: INVS (Institut de Veille Sanitaire); 2007.
  16. Cvetnic Z, Spicic S, Curic S, Jukic B, Lojkic M, Albert D, Thiebaud M, Garin-Bastuji B: g class="a-plus-plus">Isolation of Brucella suis biovar 3 from horses in Croatia.g> / Vet Rec 2005,g class="a-plus-plus">156g>(18)g class="a-plus-plus">:g>584-85.
  17. Nielsen K: g class="a-plus-plus">Diagnosis of brucellosis by serology.g> / Vet Microbiol 2002,g class="a-plus-plus">90g>(1-)g class="a-plus-plus">:g>447-59. g/10.1016/S0378-1135(02)00229-8">CrossRef
  18. Godfroid J, Nielsen K, Saegerman C: g class="a-plus-plus">Diagnosis of brucellosis in livestock and wildlife.g> / Croat Med J 2010,g class="a-plus-plus">51g>(4)g class="a-plus-plus">:g>296-05. g/10.3325/cmj.2010.51.296">CrossRef
  19. Al Dahouk S, Nockler K, Tomaso H, Splettstoesser WD, Jungersen G, Riber U, Petry T, Hoffmann D, Scholz HC, Hensel A, Neubauer H: g class="a-plus-plus">Seroprevalence of brucellosis, tularemia, and yersiniosis in wild boars (Sus scrofa) from north-eastern Germany.g> / J Vet Med B Infect Dis Vet Public Health 2005,g class="a-plus-plus">52g>(10)g class="a-plus-plus">:g>444-55. g/10.1111/j.1439-0450.2005.00898.x">CrossRef
  20. Garin-Bastuji B, Hars J, Calvez D, Thiébaud M, Artois M: g class="a-plus-plus">Brucellose du porc domestique et du sanglier sauvage à Brucella suis biovar 2 en France.g> / Epidémiol et santé anim 2000, g class="a-plus-plus">38:g>1-.
  21. Ruiz-Fons F, Vicente J, Vidal D, Hofle U, Villanua D, Gauss C, Segales J, Almeria S, Montoro V, Gortazar C: g class="a-plus-plus">Seroprevalence of six reproductive pathogens in European wild boar (Sus scrofa) from Spain: the effect on wild boar female reproductive performance.g> / Theriogenology 2006,g class="a-plus-plus">65g>(4)g class="a-plus-plus">:g>731-43. g/10.1016/j.theriogenology.2005.07.001">CrossRef
  22. Rossi S, Fromont E, Artois M, Gauthier D, Hars J, Baudet E, Brandt S, Vassant J, Crucières C, Garin-Bastuji B: / étude d’un réservoir sauvage: dynamique de deux infections chez le sanglier (Sus scrofa). In:Rapport scientifique.Office National de la Chasse et de la Faune Sauvage. 2004.
  23. Bricker BJ: g class="a-plus-plus">PCR as a diagnostic tool for brucellosis.g> / Vet Microbiol 2002,g class="a-plus-plus">90g>(1-)g class="a-plus-plus">:g>435-46. g/10.1016/S0378-1135(02)00228-6">CrossRef
  24. Ouahrani-Bettache S, Soubrier MP, Liautard JP: g class="a-plus-plus">IS6501-anchored PCR for the detection and identification of Brucella species and strains.g> / J Appl Bacteriol 1996,g class="a-plus-plus">81g>(2)g class="a-plus-plus">:g>154-60. g/10.1111/j.1365-2672.1996.tb04493.x">CrossRef
  25. Hinic V, Brodard I, Thomann A, Holub M, Miserez R, Abril C: g class="a-plus-plus">IS711-based real-time PCR assay as a tool for detection of Brucella spp. in wild boars and comparison with bacterial isolation and serology.g> / BMC Vet Res 2009., g class="a-plus-plus">5:g>
  26. Navarro E, Casao MA, Solera J: g class="a-plus-plus">Diagnosis of human brucellosis using PCR.g> / Expert Rev Mol Diagn 2004,g class="a-plus-plus">4g>(1)g class="a-plus-plus">:g>115-23. g/10.1586/14737159.4.1.115">CrossRef
  27. O'Leary S, Sheahan M, Sweeney T: g class="a-plus-plus">Brucella abortus detection by PCR assay in blood, milk and lymph tissue of serologically positive cows.g> / Res Vet Sci 2006,g class="a-plus-plus">81g>(2)g class="a-plus-plus">:g>170-76. g/10.1016/j.rvsc.2005.12.001">CrossRef
  28. Romero C, Pardo M, Grillo MJ, Diaz R, Blasco JM, Lopez-Goni I: g class="a-plus-plus">Evaluation of PCR and indirect enzyme-linked immunosorbent assay on milk samples for diagnosis of brucellosis in dairy cattle.g> / J Clin Microbiol 1995,g class="a-plus-plus">33g>(12)g class="a-plus-plus">:g>3198-200.
  29. Fredriksson-Ahomaa M, Wacheck S, Koenig M, Stolle A, Stephan R: g class="a-plus-plus">Prevalence of pathogenic Yersinia enterocolitica and Yersinia pseudotuberculosis in wild boars in Switzerland.g> / Int J Food Microbiol 2009,g class="a-plus-plus">135g>(3)g class="a-plus-plus">:g>199-02. g/10.1016/j.ijfoodmicro.2009.08.019">CrossRef
  30. Godfroid J, Saegerman C, Wellemans V, Walravens K, Letesson J-J, Tibor A, Mc Millan A, Spencer S, Sanna M, Bakker D, / et al.: g class="a-plus-plus">How to substantiate eradication of bovine brucellosis when aspecific serological reactions occur in the course of brucellosis testing.g> / Vet Microbiol 2002,g class="a-plus-plus">90g>(1-)g class="a-plus-plus">:g>461-77. g/10.1016/S0378-1135(02)00230-4">CrossRef
  31. Gyuranecz M, Erdélyi K, Makrai L, Fodor L, Szépe B, Mészáros AR, Dán A, Dencso L, Fassang E, Szeredi L: g class="a-plus-plus">Brucellosis of the European brown hare (Lepus europaeus).g> / J Comp Pathol 2011,g class="a-plus-plus">145g>(1)g class="a-plus-plus">:g>1-. g/10.1016/j.jcpa.2010.11.013">CrossRef
  32. Klein F: g class="a-plus-plus">Estimating growth rate in wild boar (Sus scrofa) using capture-mark-recapture data.g> In / Symposium international sur le sanglier. Toulouse (France); 1984:58-7.
  33. Limet JN, Kerkhofs P, Wijffels R, Dekeyser P: g class="a-plus-plus">Le diagnostic sérologique de la brucellose bovine par ELISA.g> / Ann Med Vet 1988,g class="a-plus-plus">132g>(7)g class="a-plus-plus">:g>565-75.
  34. World Organisation for Animal Health (OIE): / Chapter 2.4.3 Bovine Brucellosis. InManual for Diagnosis Tests and Vaccines for Terrestrial Animals. 2011. access online. g/Health_standards/tahm/2.04.03_BOVINE_BRUCELL.pdf" class="a-plus-plus">http://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/2.04.03_BOVINE_BRUCELL.pdf
  
• 作者单位：Fabien Grégoire (1)
  Bénédicte Mousset (1)
  David Hanrez (1)
  Charles Michaux (2)
  Karl Walravens (3)
  Annick Linden (1)
  
  1. Surveillance Network of Wildlife Diseases, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, 4000, Liège, Belgium
  2. Unit of Bioinformatics, Department of Animal Sciences, Faculty of Veterinary Medicine, University of Liège, 4000, Liège, Belgium
  3. Department of Bacteriology and Immunology, Veterinary and Agrochemical Research Centre (VAR-CODA-CERVA), 1180, Brussels, Belgium
  

文摘

Background Brucellosis is frequently reported among wild boar populations in Europe. The aim of the study was to assess the epidemiological situation in Belgium, regarding the steady increase of wild boar populations over the last decades. Several serological tests were used and compared with culture and IS711 polymerase chain reaction (PCR), to determine the most suitable combination of diagnostic tools for conducting a successful prevalence study in wildlife. Results An indirect enzyme-linked immunosorbent assay (iELISA) was used on 1168 sera from hunter-killed wild boar sampled between 2003 and 2007 in 4 natural regions of southern Belgium. Results gave an apparent prevalence of 54.88% (95% CI 52.03-57.73). Prevalence was significantly affected by age and by the year of study, but not by sex nor by the region of sampling. The relative sensitivities of the complement fixation test (CFT), the Rose Bengal test (RBT), and the slow agglutination test (SAT) versus the iELISA differed widely between tests, reaching 62.67%, 46.68%, and 34.77%, respectively. The relative specificities of the CFT, RBT and SAT versus the iELISA were respectively 99.01%, 92.49%, and 99.1%. From seropositive animals (iELISA), 9% were positive by culture and 24% by PCR when testing spleen and/or tonsils. Sensitivity of the PCR was higher on tonsils than on spleen. All bacterial isolates were identified as Brucella suis biovar 2. Conclusions Brucellosis is widespread among wild boar in southern Belgium, with seroprevalences having increased over ten years, and constitutes a growing risk of spillback to outdoor-farmed pig herds. The iELISA showed a better sensitivity than the CFT, RBT and SAT. Serological tests must be associated with direct diagnosis and PCR proved more sensitive than culture under wildlife sampling conditions. Spleen and tonsils are lymphoid tissues usually sampled in multi-disease monitoring programs. They remain top-grade organs for direct diagnosis of brucellosis, with a preference for tonsils.