Comparison of ectoine synthesis regulation in secreting and non-secreting strains of Halomonas
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- 作者：Shuang Gao (1)
Linghua Zhang (1)
Danni Li (1)
Sha Liu (1)
Xiaolin Li (1) - 关键词：Ectoine ; secreting strain ; Release kinetics ; Uptake kinetics ; Recombinant expression of teaA
- 刊名：Annals of Microbiology
- 出版年：2014
- 出版时间：September 2014
- 年：2014
- 卷：64
- 期：3
- 页码：1357-1361
- 全文大小：373 KB
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13. Zhang LH, Lang YJ, Nagata S (2009) Efficient production of ectoine using ectoine-excreting strain. Extremophiles 13:717鈥?24 CrossRef - 作者单位：Shuang Gao (1)
Linghua Zhang (1)
Danni Li (1)
Sha Liu (1)
Xiaolin Li (1)
1. Environmental Science and Engineering College, Dalian Maritime University, 1 Linghai Road, Dalian, 116026, People鈥檚 Republic of China - ISSN：1869-2044
文摘
Ectoine is an osmotic pressure compatible solute. It is synthesized by Halomonas and other microorganisms in a hypertonic environment. As a stabilizing agent of cells proteins, nucleic acids and other biological products, ectoine has wide applications. Therefore, an efficient production method for ectoine is in great demand. Ectoine is overproduced by Halomonas salina DSM 5928, an ectoine-secreting strain, in which the synthesis of ectoine is not limited by its intracellular threshold concentration. In order to explain the mechanism of secretion of ectoine, the response to NaCl stress, and the release and uptake kinetics of ectoine were compared between H. salina DSM 5928 and Halomonas elongata DSM 2581, a non-ectoine-secreting strain. Moreover, the ectoine binding protein TeaA from each of these two strains was cloned and expressed, and binding abilities were examined in vitro. The results indicated that H. salina DSM 5928 and H. elongata DSM 2581 respond to NaCl in the medium in different ways. Compared with H. elongata DSM 2581, the amount of ectoine released was higher and the uptake of ectoine under NaCl stress was lower in H. salina DSM 5928. In addition, the binding ability of TeaA to ectoine in H. salina DSM 5928 was also lower. These results reveal the secretion mechanism of ectoine as well as critical regulation and control factors involved in ectoine synthesis.