Regeneration of buckwheat plantlets from hypocotyl and the influence of exogenous hormones on rutin content and rutin biosynthetic gene expression in vitro
详细信息    查看全文
  • 作者:Siyu Hou ; Zhaoxia Sun ; Bin Linghu
  • 关键词:F. tataricum ; F. esculentum ; Rutin ; Plant hormones ; In vitro
  • 刊名:Plant Cell, Tissue and Organ Culture
  • 出版年:2015
  • 出版时间:March 2015
  • 年:2015
  • 卷:120
  • 期:3
  • 页码:1159-1167
  • 全文大小:1,198 KB
  • 参考文献:1. Chen, LH, Xu, ZQ (2006) Tissue culture and high-frequency plant regeneration of buckwheat (Fagopyrum esculentum Moench). J Mol Cell Biol 39: pp. 445-452
    2. Christa, K, Soral-mietana, M (2008) Buckwheat grains and buckwheat products-nutritional and prophylactic value of their components. Czech J Food Sci 26: pp. 153-162
    3. Cominelli, E, Gusmaroli, G, Allegra, D, Galbiati, M, Wade, HK, Jenkins, GI, Tonelli, C (2008) Expression analysis of anthocyanin regulatory genes in response to different light qualities in Arabidopsis thaliana. J Plant Physiol 165: pp. 886-894 CrossRef
    4. Ehlting, J, Buttner, D, Wang, Q, Douglas, CJ, Somssich, IE, Kombrink, E (1999) Three 4-coumarate: coenzyme A ligases in Arabidopsis thaliana represent two evolutionarily divergent classes inangiosperms. Plant J 19: pp. 9-20 CrossRef
    5. Guo, B, Han, YH, Huang, KS, Lu, Y, Hou, SY (2013) Researched on rutin contents among 30 buckwheat cultivars by HPLC. J Shanxi Agric Sci 41: pp. 26-29
    6. James, JT, Tugizimana, F, Steenkamp, PA, Dubery, IA (2013) Metabolomic analysis of Methyl Jasmonate-Induced triterpenoid production in the medicinal herb Centellaasiatica (L.) urban. Molecules 18: pp. 4267-4281 CrossRef
    7. Jiang, P, Burczynski, F, Campbell, C, Pierce, G, Austria, JA, Briggs, CJ (2007) Rutin and flavonoid contents in three buckwheat species Fagopyrum esculentum, F. tataricum and F. homotropicum and their protective effects against lipid peroxidation. Food Res Int 40: pp. 356-364 CrossRef
    8. Jin, H, Jia, JF, Hao, JG (2002) Efficient plant regeneration in vitro in buckwheat. Plant Cell Tiss Org Cult 69: pp. 293-295 CrossRef
    9. Jovanka, MD, Ne?kovi?, M, Ninkovi?, S, Crkvenjakov, R (1992) Agrobacterium-mediated transformation and plant regeneration of buckwheat (Fagopyrum esculentum Moench). Plant Cell Tiss Org Cult 29: pp. 101-108 CrossRef
    10. Kim, HJ, Park, KJ, Lim, JH (2011) Metabolomic analysis of phenolic compounds in buckwheat (Fagopyrum esculentum M.) sprouts treated with methyl jasmonate. J Agric Food Chem 59: pp. 5707-5713 CrossRef
    11. Kreft, S, Knapp, M, Kreft, I (1999) Extraction of rutin from buckwheat (Fagopyrum esculentum Moench) seeds and determination by capillary electrophoresis. J Agric Food Chem 47: pp. 4649-4652 CrossRef
    12. Kreft, I, Fabjan, N, Yasumoto, K (2006) Rutin content in buckwheat (Fagopyrum esculentum Moench) food materials and products. Food Chem 98: pp. 508-512 CrossRef
    13. Lee, SY, Kim, YK, Uddin, MR, Park, NI, Park, SU (2009) An efficient protocol for shoot organogenesis and plant regeneration of buckwheat (Fagopyrum esculentum Moench.). Rom Biotechnol Lett 14: pp. 4524-4529
    14. Lee, YJ, Kim, KJ, Park, KJ, Yoon, BR, Lim, JH, Lee, OH (2013) Buckwheat (Fagopyrum esculentum M.) sprout treated with methyl jasmonate (MeJA) improved anti-adipogenic activity associated with the oxidative stress system in 3T3-L1 adipocytes. Int J Mol Sci 14: pp. 1428-1442 CrossRef
    15. Livak, KJ, Schmittgen, TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2?ΔΔct method. Methods 25: pp. 402-408 CrossRef
    16. Mendler-Drienyovszki, N, Cal, AJ, Dobránszki, J (2013) Progress and prospects for interspecific hybridization in buckwheat and the genus Fagopyrum. Biotechnol Adv 31: pp. 1768-1775 CrossRef
    17. Metraux, JP (2002) Recent breakthroughs in the study of salicylic acid biosynthesis. Trends Plant Sci 7: pp. 332-334
文摘
We developed an in vitro regeneration system using the buckwheat species, Fagopyrum esculentum (Wenshatianqiao, WS) and Fagopyrum tataricum (Jiujiangkuqiao, JJ) in order to test the relationship between biosynthesis and accumulation of rutin and expression of genes encoding rutin biosynthetic enzymes. Hypocotyl explants were incubated on MS solid medium supplemented with 2,4-D and different concentrations of BA to optimize callus production from ‘WS-and ‘JJ- respectively. We also compared the rutin content in F. esculentum and F. tataricum plantlets regenerating with and without BA, after treatment with salicylic acid (SA) and methyl jasmonate (MeJA) for 12, 24, 48?h. The highest rutin content (5.01?mg?gFW?) in regenerating plantlets was found in F. tataricum treated with SA for 24?h. We found no significant accumulation of rutin in F. esculentum. Following qRT-PCR analysis, gene expression patterns of rutin-biosynthesis-related genes regulated by SA and MeJA were found to be significantly different between the two buckwheat species. Four genes (FtCHS, Ft4CL, FtF3H and FtFLS) were up-regulated at different time points in ‘JJ-following application of BA, SA, and MeJA, especially after 24?h MeJA treatment, where the FtCHS expression level was 11.55 times that of control. In ‘WS-following the same treatment regime only two genes, FeCHS (2.67-fold) and Fe4CL (2.42-fold), were significantly up-regulated by MeJA and only at the latest time point (48?h); results for other genes and treatments were found to be close to or even lower than the control. These results provide an improved method for F. esculentum regeneration, extend the process to F. tataricum, and suggest that, although the gene expression patterns were quite different in F. esculentum and. F. tataricum, rutin content could be enhanced to a great extent by exogenous hormones.

© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号

地址:北京市海淀区学院路29号 邮编:100083

电话:办公室:(+86 10)66554848;文献借阅、咨询服务、科技查新:66554700