Inhibition of mammalian target of rapamycin improves neurobehavioral deficit and modulates immune response after intracerebral hemorrhage in rat

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• 作者：Qin Lu (21) (22)
  Lu Gao (21) (22)
  Lijie Huang (21) (22)
  Linhui Ruan (21) (22)
  Jianjing Yang (21) (22)
  Weilong Huang (21) (22)
  Zhenxing Li (21) (22)
  Yongliang Zhang (21)
  Kunlin Jin (23)
  Qichuan Zhuge (21) (22)
  
• 关键词：ICH ; mTOR ; Rapamycin ; Outcome ; Immune response
• 刊名：Journal of Neuroinflammation
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：11
• 期：1
• 全文大小：1,188 KB
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• 作者单位：Qin Lu (21) (22)
  Lu Gao (21) (22)
  Lijie Huang (21) (22)
  Linhui Ruan (21) (22)
  Jianjing Yang (21) (22)
  Weilong Huang (21) (22)
  Zhenxing Li (21) (22)
  Yongliang Zhang (21)
  Kunlin Jin (23)
  Qichuan Zhuge (21) (22)
  
  21. Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China
  22. Zhejiang Provincial Key Laboratory of Aging and Neurological Disease, Wenzhou Medical University, Wenzhou, Zhejiang, China
  23. Department of Pharmacology and Neuroscience, Institute for Aging and Alzheimer’s Disease Research, University of North Texas Health Science Center at Fort Worth, Fort Worth, TX, USA
  
• ISSN：1742-2094

文摘

Background Mammalian target of rapamycin (mTOR), a serine/threonine kinase, regulates many processes, including cell growth and the immune response. mTOR is also dysregulated in several neurological diseases, such as traumatic brain injury (TBI), stroke, and neurodegenerative disease. However, the role of mTOR in intracerebral hemorrhage (ICH) remains unexplored. The aims of our study were to determine whether inhibiting mTOR signaling could affect the outcome after ICH and to investigate the possible underlying mechanism. Methods A rat ICH model was induced by intracerebral injection of collagenase IV into the striatum, and mTOR activation was inhibited by administration of rapamycin. mTOR signaling activation was determined by western blotting. Neurobehavioral deficit after ICH was determined by a set of modified Neurological Severity Scores (mNSS). The levels of CD4+CD25+Foxp3+ regulatory T cells (Tregs) and cytokines were examined using flow cytometry and ELISA, respectively. Results Our results demonstrated thatmTOR signaling was activated 30 minutes and returned to its basal level 1 day after ICH. Increased p-mTOR, which mean that mTOR signaling was activated, was predominantly located around the hematoma. Rapamycin treatment significantly improved the neurobehavioral deficit after ICH, increased the number of Tregs, increased levels of interleukin-10 and transforming growth factor-β and reduced interferon-γ both in peripheral blood and brain. Conclusions Our study suggests that mTOR improves ICH outcome and modulates immune response after ICH.