Enhanced validamycin production and gene expression at elevated temperature in Streptomyces hygroscopicus subsp. jingangensis 5008
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- 作者：Lei Li (1)
LinQuan Bai (1)
XiuFen Zhou (1)
ZiXin Deng (1) - 关键词：Streptomyces ; validamycin ; regulation ; enhanced production
- 刊名：Chinese Science Bulletin
- 出版年：2009
- 出版时间：April 2009
- 年：2009
- 卷：54
- 期：7
- 页码：1204-1209
- 全文大小：756KB
- 参考文献：1. Demain A L. Microbial biotechnology. Trends Biotechnol, 2000, 18: 26-1 CrossRef
2. Bentley S D, Chater K F, Cerdeno-Tarraga A M, et al. Complete genome sequence of the model actinomycete / Streptomyces coelicolor A3(2). Nature, 2002, 417: 141-47 CrossRef
3. Ikeda H, Nonomiya T, Usami M, et al. Organization of the biosynthetic gene cluster for the polyketide anthelmintic macrolide avermectin in / Streptomyces avermitilis. Proc Natl Acad Sci USA, 1999, 96: 9509-514 CrossRef
4. Oliynyk M, Samborskyy M, Lester J B, et al. Complete genome sequence of the erythromycin-producing bacterium / Saccharopolyspora erythraea NRRL23338. Nat Biotechnol, 2007, 25: 447-53 CrossRef
5. Tahlan K, Ahn S K, Sing A, et al. Initiation of actinorhodin export in / Streptomyces coelicolor. Mol Microbiol, 2007, 63: 951-61 CrossRef
6. Sola-Landa A, Moura R S, Martin J F. The two-component PhoRPhoP system controls both primary metabolism and secondary metabolite biosynthesis in / Streptomyces lividans. Proc Natl Acad Sci USA, 2003, 100: 6133-138 CrossRef
7. Oh S Y, Shin J H, Roe J H. Dual role of OhrR as a repressor and an activator in response to organic hydroperoxides in / Streptomyces coelicolor. J Bacteriol, 2007, 189: 6284-292 CrossRef
8. Deeble V J, Lindley H K, Fazeli M R, et al. Depression of streptomycin production by / Streptomyces griseus at elevated growth temperature: studies using gene fusions. Microbiology, 1995, 141(Pt 10): 2511-518 CrossRef
9. Iwasa T, Yamamoto H, Shibata M. Studies on validamycins, new antibiotics. I. / Streptomyces hygroscopicus var. / limoneus nov. var., validamycin-producing organism. J Antibiot (Tokyo), 1970, 23: 595-02
10. Jian X, Pang X, Yu Y, et al. Identification of genes necessary for jinggangmycin biosynthesis from / Streptomyces hygroscopicus 10-2. Antonie Van Leeuwenhoek, 2006, 90: 29-9 CrossRef
11. Singh D, Seo M J, Kwon H J, et al. Genetic localization and heterologous expression of validamycin biosynthetic gene cluster isolated from / Streptomyces hygroscopicus var. / limoneus KCCM 11405 (IFO 12704). Gene, 2006, 376: 13-3 CrossRef
12. Yu Y, Bai L, Minagawa K, et al. Gene cluster responsible for validamycin biosynthesis in / Streptomyces hygroscopicus subsp. / jinggangensis 5008. Appl Environ Microbiol, 2005, 71: 5066-076 CrossRef
13. Bai L, Li L, Xu H, et al. Functional analysis of the validamycin biosynthetic gene cluster and engineered production of validoxylamine A. Chem Biol, 2006, 13: 387-97 CrossRef
14. Tarutina M, Ryjenkov D A, Gomelsky M. An unorthodox bacteriophytochrome from / Rhodobacter sphaeroides involved in turnover of the second messenger c-di-GMP. J Biol Chem, 2006, 281: 34751-4758 CrossRef
15. Xia T H, Jiao R S. Studies on glutamine synthetase from / Streptomyces hygroscopicus var. / jinggangensis (In Chinese). Sci Sin, 1986, 29: 379-88
16. Kieser T, Bibb M J, Chater K F, et al. Practical Streptomyces genetics. A Laboratory Manual. Norwich: The John Innes Foundation, 2000
17. Paget M S, Chamberlin L, Atrih A, et al. Evidence that the extracytoplasmic function sigma factor sigmaE is required for normal cell wall structure in / Streptomyces coelicolor A3(2). J Bacteriol, 1999, 181: 204-11
18. Moffatt B A, Studier F W. T7 lysozyme inhibits transcription by T7 RNA polymerase. Cell, 1987, 49: 221-27 CrossRef
19. Yanisch-Perron C, Vieira J, Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene, 1985, 33: 103-19 CrossRef
20. Smokvina T, Mazodier P, Boccard F, et al. Construction of a series of pSAM2-based integrative vectors for use in actinomycetes. Gene, 1990, 94: 53-9 CrossRef
21. Anton N, Mendes M V, Martin J F, et al. Identification of PimR as a positive regulator of pimaricin biosynthesis in / Streptomyces natalensis. J Bacteriol, 2004, 186: 2567-575 CrossRef
22. Gust B, Challis G L, Fowler K, et al. PCR-targeted / Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin. Proc Natl Acad Sci USA, 2003, 100: 1541-546 CrossRef
23. He J, Chen S W, Ruan L F, et al. Determination of the fungicide validamycin A by capillary zone electrophoresis with indirect UV detection. J Agric Food Chem, 2003, 51: 7523-527 CrossRef
24. van Schaik W, Tempelaars M H, Zwietering M H, et al. Analysis of the role of RsbV, RsbW and RsbY in regulating sigmaB activity in / Bacillus cereus. J Bacteriol, 2005, 187: 5846-851 CrossRef
25. Cho Y H, Lee E J, Ahn B E, et al. SigB, an RNA polymerase sigma factor required for osmoprotection and proper differentiation of / Streptomyces coelicolor. Mol Microbiol, 2001, 42: 205-14 CrossRef - 作者单位：Lei Li (1)
LinQuan Bai (1)
XiuFen Zhou (1)
ZiXin Deng (1)
1. School of Life Science & Biotechnology and Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, 200030, China - ISSN：1861-9541
文摘
Cultivation shift from 30° to 37° significantly enhanced validamycin (VAL) production. Analyzed by reverse-transcription PCR, the transcription of three val genes, valA, valK and valG, representing the three operons of the cluster was simultaneously increased at elevated temperature. Furthermore, the transcription of valP and valQ, a pair of two-component regulators in validamycin biosynthetic gene cluster, was also increased at 37°. Inactivation of valP and valQ reduced validamycin production at 37° to the yield level of wild type strain at 30°, and the val genes showed reduced expression in the mutant LL-8 at 37°. These results revealed that the two-component regulator valP and valQ contribute to the elevated validamycin production.