New Insights into Lidocaine and Adrenaline Effects on Human Adipose Stem Cells
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- 作者：Anne-Claire Girard (1) (2)
Michael Atlan (3)
Karima Bencharif (1) (2)
Manoj Kumar Gunasekaran (2)
Pierre Delarue (4)
Olivier Hulard (5)
Christian Lefebvre-d’Hellencourt (2)
Regis Roche (1) (2)
Laurence Hoareau (1) (2)
Franck Festy (1) (2) - 关键词：Adipose stem cells ; Adrenaline ; Fat grafting ; Lidocaine ; Lipoaspiration
- 刊名：Aesthetic Plastic Surgery
- 出版年：2013
- 出版时间：February 2013
- 年：2013
- 卷：37
- 期：1
- 页码：144-152
- 全文大小：515KB
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Michael Atlan (3)
Karima Bencharif (1) (2)
Manoj Kumar Gunasekaran (2)
Pierre Delarue (4)
Olivier Hulard (5)
Christian Lefebvre-d’Hellencourt (2)
Regis Roche (1) (2)
Laurence Hoareau (1) (2)
Franck Festy (1) (2)
1. ADIP’sculpt, Plateforme CYROI, 2 rue Maxime Rivière, Sainte Clotilde, 97490, Reunion Island, France
2. Groupe d’Etude sur l’Inflammation Chronique et l’Obésité (GEICO), Plateforme CYROI, Université de la Réunion, Saint-Denis de la Réunion, France
3. Centre Hospitalier Général de Pontoise/René Dubos, Inserm, 698, France
4. Clinique Saint Vincent, Groupe Clinifutur, Reunion Island, France
5. Clinique Les Orchidées, Groupe Clinifutur, Reunion Island, France - ISSN：1432-5241
文摘
Background Adipose stem cells have gained great interest in plastic and reconstructive surgery with their ability to improve engraftment after fat transfer for soft tissue filling. It is therefore essential to know the effect of the drugs commonly used during the lipoaspiration procedure, such as lidocaine and adrenaline. Indeed, these drugs are infiltrated at the fat donor site for local anesthesia and for reduction of bleeding. This study analyzed the effects of these drugs on the viability of adipose-derived stem cells and on their inflammatory status. Methods Adipose-derived stem cells from lipoaspirates were grown in culture before being treated with different clinical doses of lidocaine at different times of exposure (1-4?h), and with adrenaline (1?μg/mL). Cytotoxicity was measured by lactate dehydrogenase assay and by flow cytometry with annexin V/propidium iodide staining. In parallel, the secretion of the proinflammatory cytokines tumor necrosis factor-alpha (TNFα), interleukin-6 (IL-6), and monocyte chemotactic protein-1 (MCP-1) was tested by enzyme-linked immunoassay. Results Lidocaine affected cell viability after 24?h, even when the cells were exposed for only 1 or 2?h. Apoptosis was not involved in lidocaine cytotoxicity. Regarding inflammation, no TNFα was produced, and lidocaine decreased the levels of IL-6 and MCP-1 in a dose-dependent manner. In contrast, adrenaline did not influence cell viability or cytokine secretions. Conclusions Adipose tissue should be handled appropriately to remove lidocaine and adrenaline, with such procedures as washing and centrifugation. This study provides new insights into the use of lidocaine and adrenaline for fat transfer or stem cell isolation from lipoaspirates. Level of Evidence II This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.