Adsorption kinetics of low-density lipoproteins with Langmuir monolayer

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• 作者：Ziad Khattari
• 关键词：Langmuir monolayer ; Atherosclerosis ; LDL oxidation ; Adsorption kinetics
• 刊名：Journal of Biological Physics
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：42
• 期：4
• 页码：539-550
• 全文大小：1,248 KB
• 刊物类别：Physics and Astronomy
• 刊物主题：Physics
  Biophysics and Biomedical Physics
  Condensed Matter
  Statistical Physics
  Polymer Sciences
  Bioinformatics
  Neurosciences
  
• 出版者：Springer Netherlands
• ISSN：1573-0689
• 卷排序：42

文摘

The present work utilizes the Langmuir monolayer technique to detect the adsorption kinetics of native low-density lipoproteins and their oxidized form with the lipid monolayer. We found that low-density lipoproteins and oxidized low-density lipoproteins are able to penetrate the LM up to pressure π = 9.9 and 11.6 mN/m. Also, the adsorption constants of both particles were found to depend strongly on the monolayer initial pressure. It is found that less compressed lipid monolayers could accommodate more native low-density lipoproteins than the oxidized ones due their higher binding affinity toward monolayers. The probable α-helical regions along the apoproteinB-100 secondary structure and average hydrophobicity could explain partially their adsorption kinetics into lipid monolayers. This simplified ‘in vitro’ study of low-density lipoprotein–monolayer interaction may serve as a step further to understand the mechanism and bioactivity of the atherosclerotic process. Also, it may shed light on the oxidized low-density lipoprotein’s role in plaque formation in the innermost arterial wall in blood vessels.