Chromatin reorganization and endogenous auxin/cytokinin dynamic activity during somatic embryogenesis of cultured cotton cell
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- 作者：Fanchang Zeng ; Xianlong Zhang ; Shuangxia Jin ; Lei Cheng ; Shaoguang Liang ; Lisong Hu ; Xiaoping Guo ; Yichun Nie and Jinglin Cao
- 关键词：Chromatin reorganization ; Dynamic activity ; Endogenous auxin/cytokinin ; Flow cytometry ; Gossypium hirsutum ; Somatic embryogenesis
- 刊名：Plant Cell, Tissue and Organ Culture
- 出版年：2007
- 出版时间：July, 2007
- 年：2007
- 卷：90
- 期：1
- 页码：63-70
- 全文大小：263 KB
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Plant Sciences
Plant Physiology - 出版者：Springer Netherlands
- ISSN：1573-5044
文摘
We conducted a systematic assessment and comparative study on the biochemical and cellular characteristics of cultured cotton cells during the entire process of somatic embryogenesis (SE). All staged cultures were widely investigated in this assay. Cell and tissue ectogenesis manipulation combined with flow cytometry (FCM) was employed to cellular study during the whole totipotency process of dedifferentiation and redifferentiation. We identified two phases of chromatin decondensation during the dedifferentiation and redifferentiation. At the same time, sharp increase in the ratio of indoleacetic acid (IAA), isopentenyladenosine group (iPAs) at the same stage of cell dedifferentiation and redifferentiation process serve as distinct biochemical maker of dedifferentiation and SE initiation with the unique feature. Our results suggest the two phases of chromatin reorganization associated with endogenous auxin/cytokinin dynamic activity may underlie dedifferentiation and redifferentiation during the entire SE process in cotton.