Enterocin B3A-B3B produced by LAB collected from infant faeces: potential utilization in the food industry for Listeria monocytogenes biofilm management
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- 作者：Alaa Al-Seraih ; Yanath Belguesmia ; John Baah ; Sabine Szunerits…
- 关键词：Class IIb Enterocin B3A ; B3B ; Nisin ; Listeria monocytogenes ; Resistance ; Biofilm
- 刊名：Antonie van Leeuwenhoek
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：110
- 期：2
- 页码：205-219
- 全文大小：
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Microbiology; Medical Microbiology; Plant Sciences; Soil Science & Conservation;
- 出版者：Springer International Publishing
- ISSN：1572-9699
- 卷排序：110
文摘
Enterococcus faecalis B3A-B3B produces the bacteriocin B3A-B3B with activity against Listeria monocytogenes, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA) and Clostridium perfringens, but apparently not against fungi or Gram-negative bacteria, except for Salmonella Newport. B3A-B3B enterocin has two different nucleotides but similar amino acid composition to the class IIb MR10A-MR10B enterocin. B3A-B3B consists of two peptides of predicted molecular mass of 5176.31 Da (B3A) and 5182.21 Da (B3B). Importantly, B3A-B3B impeded biofilm formation of the foodborne pathogen L. monocytogenes 162 grown on stainless steel. The antimicrobial treatment of stainless steel with nisin (1 or 16 mg ml−1) decreased the cell numbers by about 2 log CFU ml−1, thereby impeding the biofilm formation by L. monocytogenes 162 or its nisin-resistant derivative strain L. monocytogenes 162R. Furthermore, the combination of nisin and B3A-B3B enterocin reduced the MIC required to inhibit this pathogen grown in planktonic or biofilm cultures.