Exogenous Interleukin-6 Facilitated the Contraction of the Colon in a Depression Rat Model
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- 作者：Li Zhang (1)
Liudan Hu (1)
Mingkai Chen (1)
Baoping Yu (1) - 关键词：Interleukin ; 6 ; CUMS ; Colon motility ; Depression
- 刊名：Digestive Diseases and Sciences
- 出版年：2013
- 出版时间：August 2013
- 年：2013
- 卷：58
- 期：8
- 页码：2187-2196
- 全文大小：706KB
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Liudan Hu (1)
Mingkai Chen (1)
Baoping Yu (1)
1. Department of Gastroenterology, Renmin Hospital of Wuhan University, Number 238, Jiefang Road, Wuhan, 430060, Hubei Province, China
文摘
Background Gut dysmotility is closely associated with proinflammatory cytokines both in irritable bowel syndrome and inflammatory bowel disease. There is a dose–response relationship between depression and these inflammatory cytokines. Aims In the present study, we aimed to investigate the effect of Interleukin-6 (IL-6) on colon motility in a rat model of depression induced by chronic unpredictable mild stress (CUMS). Methods The contraction of the circular muscle strips of proximal colon was monitored by a polygraph. IL-6 and IL-6 receptor (IL-6R) mRNA was assayed by real-time quantitative PCR. Immunohistochemistry staining was used to locate the IL-6 and IL-6R in the rat colon. Results IL-6 and IL-6R were expressed in the mucosal layer, smooth muscle cells, and myenteric plexus of the colon. Exogenous IL-6 (20?ng/ml) increased the contraction of the circular muscle strip. Pretreatment of tetrodotoxin (blocker of voltage-dependent Na+ channel on nerve fiber) blocks the excitatory effect of IL-6 on the contraction of the colon in non-stressed rats, but partially inhibited IL-6-induced excitatory effect on the muscle strips in CUMS-treated rats. Conclusions These results suggest that IL-6-induced the contraction of the colonic strip by acting on the gut’s nervous system and acting directly on the smooth muscle in rats with depression.