Characterization of Enzymes in the Oxidation of 1,2-Propanediol to d-(?-Lactic Acid by Gluconobacter oxydans DSM 2003

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• 作者：Liujing Wei (1)
  Xuepeng Yang (3)
  Keliang Gao (1)
  Jinping Lin (1)
  Shengli Yang (2)
  Qiang Hua (2)
  Dongzhi Wei (1)
  
• 关键词：Gluconobacter oxydans ; Alcohol dehydrogenase ; Aldehyde dehydrogenase ; 1 ; 2 ; Propanediol ; d ; (? ; lactic acid
• 刊名：Molecular Biotechnology
• 出版年：2010
• 出版时间：September 2010
• 年：2010
• 卷：46
• 期：1
• 页码：26-33
• 全文大小：324KB
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• 作者单位：Liujing Wei (1)
  Xuepeng Yang (3)
  Keliang Gao (1)
  Jinping Lin (1)
  Shengli Yang (2)
  Qiang Hua (2)
  Dongzhi Wei (1)
  
  1. State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China
  3. College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, 450002, China
  2. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China
  

文摘

Although Gluconobacter oxydans can convert 1,2-propanediol to d-(?-lactic acid, the enzyme(s) responsible for the conversion has remain unknown. In this study, the membrane-bound alcohol dehydrogenase (ADH) of Gluconobacter oxydans DSM 2003 was purified and confirmed to be essential for the process of d-(?-lactic acid production by gene knockout and complementation studies. A 25 percent decrease in d-(?-lactic acid production was found for the aldehyde dehydrogenase (ALDH) deficient strain of G. oxydans DSM 2003, indicating that this enzyme is involved in the reaction but not necessary. It is the first report that reveals the function of ADH and ALDH in the biooxidation of 1,2-propanediol to d-(?-lactic acid by G. oxydans DSM 2003.