Preinduction of heat shock protein 70 protects mice against post-infection irritable bowel syndrome via NF-κB and NOS/NO signaling pathways

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• 作者：Xuchun Zhou ; Liwei Dong ; Bo Yang ; Zhoutao He ; Yiyao Chen ; Taozhi Deng…
• 关键词：HSP70 ; PI ; IBS ; Inflammation ; NF ; κB ; NOS/NO ; Mice
• 刊名：Amino Acids
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：47
• 期：12
• 页码：2635-2645
• 全文大小：3,558 KB
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• 作者单位：Xuchun Zhou (1)
  Liwei Dong (2)
  Bo Yang (1)
  Zhoutao He (2)
  Yiyao Chen (2)
  Taozhi Deng (2)
  Baili Huang (2)
  Cheng Lan (2)
  
  1. Department of Gastroenterology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China
  2. Department of Gastroenterology, Hainan Provincial General Hospital, Haikou, 570311, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Analytical Chemistry
  Biochemical Engineering
  Life Sciences
  Proteomics
  Neurobiology
  
• 出版者：Springer Wien
• ISSN：1438-2199

文摘

This study aimed to investigate the protective effects of preinduction of heat shock protein 70 (HSP70) on Trichinella spiralis infection-induced post-infectious irritable bowel syndrome (PI-IBS) in mice. Trichinella spiralis infection significantly reduced HSP70 abundance, ileal villus height and crypt depth, expression of tight junctions, serum lysine and arginine concentrations, and ileal SCL7A6 and SCL7A7 mRNA levels, induced inflammatory response, and activated NF-κB signaling pathway. Meanwhile, the heat treatment upregulated HSP70 expression, and then reversed intestinal dysfunction and inflammatory response. Preinduction of HSP70 enhanced serum arginine and intestinal SCL7A7 expression and inhibited NF-κB activation compared with PI-IBS model. Treatment with pyrrolidine dithiocarbamate (PDTC, an NF-κB inhibitor) and N-nitro-l-arginine methyl ester hydrochloride (l-NAME, a nitric oxide synthase inhibitor, NOS) further demonstrated that preinduction of HSP70 might inhibit NF-κB and activated NOS/nitric oxide (NO) signaling pathways. In conclusion, preinduction of HSP70 by heat treatment may confer beneficial effects on Trichinella spiralis infection-induced PI-IBS in mice, and the protective effect of HSP70 may be associated with inhibition of NF-κB and stimulation of NOS/NO signaling pathways. Keywords HSP70 PI-IBS Inflammation NF-κB NOS/NO Mice