Drought stress condition increases root to shoot ratio via alteration of carbohydrate partitioning and enzymatic activity in rice seedlings

详细信息    查看全文

• 作者：Wei Xu (1)
  Kehui Cui (1)
  Aihui Xu (1)
  Lixiao Nie (1)
  Jianliang Huang (1)
  Shaobing Peng (1)
  
  1. National Key Laboratory of Crop Genetic Improvement ; MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River ; College of Plant Science and Technology ; Huazhong Agricultural University ; Wuhan ; 430070 ; China
  
• 关键词：Carbohydrate partitioning ; Invertase ; Rice (Oryza sativa L) ; Root to shoot ratio ; Sucrose ; phosphate synthase ; Drought stress condition
• 刊名：Acta Physiologiae Plantarum
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：37
• 期：2
• 全文大小：693 KB
• 参考文献：1. Asch F, Dingkuhn M, Sow A, Audebert A (2005) Drought-induced changes in rooting patterns and assimilate partitioning between root and shoot in upland rice. Field Crop Res 93:223鈥?36 CrossRef
  2. Azhiri-Sigari TA, Yamauchi A, Kamoshita A, Wade LJ (2000) Genotypic variation in response of rainfed lowland rice to drought would help to elucidate how rooting depth and deep root and rewatering II. Root growth. Plant Prod Sci 3:180鈥?88 CrossRef
  3. Bradford MM (1976) A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248鈥?54 CrossRef
  4. Chiou TJ, Bush DR (1998) Sucrose is a signal molecule in assimilate partitioning. P Natl Acad Sci USA 95:4784鈥?788 CrossRef
  5. Cui KH, Huang JL, Xing YZ, Yu SB, Xu CG, Peng SB (2008) Mapping QYLs for seedling characteristics under different water supply conditions in rice ( / Oryza sativa). Physiol Plant 132:53鈥?8
  6. D茅jardin A, Sokolov LN, Kleczkowski LA (1999) Sugar/osmoticum levels modulate differential abscisic acid-independent expression of two stress-responsive sucrose synthase genes in Arabidopsis. Biochem J 344:503鈥?09 CrossRef
  7. Ericsson T (1995) Growth and shoot: root ratio of seedlings in relation to nutrient availability. Plant Soil 168鈥?69:205鈥?14 CrossRef
  8. Farrar J (1996) Regulation of shoot-root ratio is mediated by sucrose. Plant Soil 185:13鈥?9 CrossRef
  9. Geigenberger P, Reimholz R, Geiger M, Merlo L, Canale V, Stitt M (1997) Regulation of sucrose and starch metabolism in potato tubers in response to short-term water deficit. Planta 201:502鈥?18 CrossRef
  10. Gowda VRP, Henry A, Yamauchi A, Shashidhar HE, Serraj R (2011) Root biology and genetic improvement for drought avoidance in rice. Field Crop Res 122:1鈥?3 CrossRef
  11. Guo L, Wang ZY, Lin H, Cui WE, Chen J, Liu MH, Chen ZhL, Qu LJ, Gu HY (2006) Expression and functional analysis of the rice plasma-membrane intrinsic protein gene family. Cell Res 16:277鈥?86 CrossRef
  12. Hammond JP, White PJ (2011) Sugar signaling in root response to low phosphorus availability. Plant Physiol 156:1033鈥?040 CrossRef
  13. Hirai G, Chujo H, Tanaka O, Okumura T, Takeuchi S (1994) Studies on the effect of relative humidity of the atmosphere on growth and physiology of rice plants. IX. Effects of water stresses induced by low humidity and the addition of polyethylene glycol to the medium on growth. Jpn J Crop Sci 63:265鈥?70 CrossRef
  14. Huber SC, Huber JL (1996) Role and regulation of sucrose phosphate synthase in higher plants. Ann Rev Plant Physiol Plant Mol Bio 47:431鈥?44 CrossRef
  15. Ji KX, Wang YY, Sun WN, Lou QJ, Mei HW, Shen SH, Chen H (2012) Drought-responsive mechanisms in rice genotypes with contrasting drought tolerance during reproductive stage. J Plant Physiol 169:336鈥?44 CrossRef
  16. Kathiresan A, Lafitte HR, Chen JX, Mansueto L, Bruskiewich R, Bennett J (2006) Gene expression microarrays and their application in drought stress research. Field Crop Res 97:102鈥?10 CrossRef
  17. Koch K (2004) Sucrose metabolism: regulatory mechanisms and pivotal roles in sugar sensing and plant development. Curr Opin Plant Biol 7:235鈥?46 CrossRef
  18. Lemoine R, Camera SL, Atanassova R, D茅dald茅champ F, Allario T, Pourtau N, Bonnemain JL, Laloi M, Coutos-Th茅venot P, Maurousset L, Faucher M, Girousse C, Lemonnier P, Parrilla J, Durand M (2013) Source-to-sink transport of sugar and regulation by environmental factors. Front Plant Sci. doi:10.3389/fpls.2013.00272
  19. Li ZM, Palmer WM, Martin AP, Wang RQ, Rainsford F, Jin Y, Patrick JW, Yang YJ, Ruan YL (2012) High invertase activity in tomato reproductive organs correlates with enhanced sucrose import into, and heat tolerance of, young fruit. J Exp Bot 63:1155鈥?166 CrossRef
  20. Liu HS, Li FM, Xu H (2004) Deficiency of water can enhance root respiration rate of drought-sensitive but not drought-tolerant spring wheat. Agr Water Manage 64:41鈥?8 CrossRef
  21. Lou Y, Gou JY, Xue HW (2007) PIP5K9, an Arabidopsis phosphatidylinositol monophosphate kinase, interacts with a cytosolic invertase to negatively regulate sugar-mediated root growth. Plant Cell 19:163鈥?81 CrossRef
  22. Lowell CA, Tomlinson PT, Koch KE (1989) Sucrose-metabolizing enzymes in transport tissues and adjacent sink structures in developing citrus fruit. Plant Physiol 90:1394鈥?402 CrossRef
  23. Mahajan S, Tuteja N (2005) Cold, salinity and drought stresses: an overview. Arch Biochem Biophy 444:139鈥?58 CrossRef
  24. Nicotra AB, Atkin OK, Bonser SP, Davidson AM, Finnegan EJ, Mathesius U, Poot P, Purugganan MD, Richards CL, Valladares F, van Kleunen M (2011) Plant phenotypic plasticity in a changing climate. Trends in Plant Sci 15:684鈥?92 CrossRef
  25. Pavlinova OA, Balakhontsev EN, Prasolova MF, Turkina MV (2002) Sucrose-phosphate synthase, sucrose synthase, and invertase in sugar beet leaves. Russ J Plant Physiol 49:69鈥?3 CrossRef
  26. Pinheiro C, Chaves MM, Ricardo CP (2001) Alterations in carbon and nitrogen metabolism induced by water deficit in the stems and leaves of / Lupinus albus L. J Exp Bot 52:1063鈥?070 CrossRef
  27. Poorter H, Niklas K, Reich PB, Oleksyn J, Poot P, Mommer L (2012) Biomass allocation to leaves, stems and roots: meta-analyses of interspecific variation and environmental control. New Phytol 193:30鈥?0 CrossRef
  28. Rich SM, Watt M (2013) Soil conditions and cereal root system architecture: review and considerations for linking Darwin and Weaver. J Exp Bot 64:1193鈥?208 CrossRef
  29. Roitsch T, Gonzalez MC (2004) Function and regulation of plant invertases: sweet sensations. Trends Plant Sci 9:606鈥?13 CrossRef
  30. Sergeeva LI, Keurentjes JJB, Bentsink L, Vonk J, van der Plas LHW, Koornneef M, Vreugdenhil D (2006) Vacuolar invertase regulates elongation of Arabidopsis thaliana roots as revealed by QTL and mutant analysis. P Natl Acad Sci USA 103:2994鈥?999 CrossRef
  31. Shone MGT, Whipps JM, Flood AV (1983) Effects of localized and overall water stress on assimilate partitioning in barley between shoots, roots and root exudates. New Phytol 95:625鈥?34 CrossRef
  32. Slama I, Messedi D, Ghnaya T, Savoure A, Abdelly C (2006) Effects of water deficit on growth and proline metabolism in / Sesuvium portulacastrum. Environ Exp Bot 56:231鈥?38 CrossRef
  33. Tang GQ, L眉scher M, Sturm A (1999) Antisense repression of vacuolar and cell wall invertase in transgenic carrot alters early plant development and sucrose partitioning. Plant Cell 11:177鈥?89 CrossRef
  34. Tripathy JN, Zhang JX, Robin S, Nguyen TT, Nguyen HT (2000) QTLs for cell-membrane stability mapped in rice ( / Oryza sativa L.) under drought stress. Theor Appl Genet 100:1197鈥?202 CrossRef
  35. Vargas WA, Pontis HG, Salerno GL (2007) Differential expression of alkaline and neutral invertases in response to environmental stresses: characterization of an alkaline isoform as a stress-response enzyme in wheat leaves. Planta 226:1535鈥?545 CrossRef
  36. Wang HG, Zhang HL, Li ZC (2007) Analysis of gene expression profile induced by water stress in upland rice ( / Oryza sativa L. var. IRAT109) seedlings using subtractive expressed sequence Tags library. J Integr Plant Biol 49:1455鈥?463 CrossRef
  37. Wilson JB (1988) A review of evidence on the control of shoot: root ratio, in relation to models. Ann Bot 61:433鈥?49
  38. Wind J, Smeekens S, Hanson J (2010) Sucrose: metabolite and signaling molecule. Phytochemistry 71:1610鈥?614 CrossRef
  39. Wissuwa M, Gamat G, Ismail AM (2005) Is root growth under phosphorus deficiency affected by source or sink limitations? J Exp Bot 56:1943鈥?950 CrossRef
  40. Yang JC, Zhang JH, Wang ZQ, Zhu QS (2001) Activities of starch hydrolytic enzymes and sucrose-phosphate synthase in the stems of rice subjected to water stress during grain filling. J Exp Bot 52:2169鈥?179
  41. Yoshida S, Forno DA, Cook JH, Gomez KA (1976) Laboratory manual for physiological studies of rice, 3rd edn. International Rice Research Institute, Manila
  42. Yue B, Xue WY, Xiong LZ, Yu XQ, Luo LJ, Cui KH, Jin DM, Xing YZ, Zhang QF (2006) Genetic basis of drought resistance at reproductive stage in rice: separation of drought tolerance from drought avoidance. Genetics 172:1213鈥?228 CrossRef
  43. Zhou J, Wang X, Jiao Y, Qin Y, Liu X, He K, Chen C, Ma L, Wang J, Xiong L, Zhang Q, Fan L, Deng XW (2007) Global genome expression analysis of rice in response to drought and high-salinity stresses in shoot, flag leaf, and panicle. Plant Mol Biol 63:591鈥?08 CrossRef
  44. Zhu YJ, Komor E, Moore PH (1997) Sucrose accumulation in the sugarcane stem is regulated by the difference between the activities of soluble acid invertase and sucrose phosphate synthase. Plant Physiol 115:609鈥?16 CrossRef
  
• 刊物主题：Plant Physiology; Plant Genetics & Genomics; Plant Biochemistry; Plant Pathology; Plant Anatomy/Development; Agriculture;
• 出版者：Springer Berlin Heidelberg
• ISSN：1861-1664

文摘

To understand the underlying mechanism for plasticity in root to shoot ratio (R/S) in response to drought stress, two rice cultivars, Zhenshan97 (drought susceptible) and IRAT109 (drought resistant), were grown hydroponically, and R/S, carbohydrate concentration and partitioning, and activities of enzymes for sucrose conversion in seedlings exposed to drought stress condition (DS) imposed by polyethylene glycol 6000 were investigated. The R/S significantly increased under DS in comparison with that under well-watered condition. The proportion of dry matter and soluble sugar of roots markedly increased under DS. The R/S was negatively correlated with proportion of soluble sugar in stems, and positively with the proportions of soluble sugar and starch in roots. Drought stress condition significantly increased leaf sucrose-phosphate synthase (EC 2.4.1.14) activity and root acid and neutral/alkaline invertase (EC 3.2.1.26) activity. The R/S was positively correlated with leaf sucrose-phosphate synthase and root acid invertase activity, and negatively with leaf sucrose synthase activity in the cleavage direction. Our results indicate that the increase in R/S in response to DS is closely associated with the higher proportion of dry matter and soluble sugar in roots, and this occurs via an increase in leaf sucrose-phosphate synthase and root invertase activity, and thus more sucrose is available for transport from leaves to roots.