Lysobacter chengduensis sp. nov. Isolated from the Air of Captive Ailuropoda melanoleuca Enclosures in Chengdu, China

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• 作者：Caifang Wen ; Lixin Xi ; Rong She ; Shan Zhao ; Zhongxiang Hao ; Lu Luo…
• 刊名：Current Microbiology
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：72
• 期：1
• 页码：88-93
• 全文大小：420 KB
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• 作者单位：Caifang Wen (1)
  Lixin Xi (1)
  Rong She (1)
  Shan Zhao (1)
  Zhongxiang Hao (1)
  Lu Luo (1)
  Hong Liao (1)
  Zhenrong Chen (1)
  Guoquan Han (2)
  Sanjie Cao (1)
  Rui Wu (1)
  Qigui Yan (1)
  Rong Hou (3)
  
  1. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China
  2. College of Food Science, Sichuan Agricultural University, Yaan, China
  3. Chengdu Research Base of Giant Panda Breeding, Chengdu, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Biotechnology
  
• 出版者：Springer New York
• ISSN：1432-0991

文摘

A novel bacterial strain, designated as CF21T, was isolated from the air of Ailuropoda melanoleuca enclosures in China. Cells were gram-negative, aerobic, non-motile, and rod shaped. Strain CF21T grew at 10–40 °C (optimum 28–30 °C) and pH 6.0–9.0 (optimum pH 7.0–8.0) and in the presence of NaCl concentrations ranging from 0.0 % (w/v) to 2.0  % (optimum 0.0–1.0 %). 16SrRNA gene sequence analysis indicated that strain CF21T belonged to genus Lysobacter within class Gammaproteobacteria and was most closely related to Luteimonas dalianensi OB44-3T (95.8 % similarity), Lysobacter ruishenii CTN-1T (95.1 %), Lysobacter spongiicola KMM329T (94.8 %), and Lysobacter daejeonensis GH1-9T (94.6 %). The genomic G+C DNA content was 68.72 mol%. Major cellular fatty acids of CF21T were iso-C_16:0 (30.22 %), iso-C_15:0 (25.70 %), and the sum of 10-methyl C_16 : 0 and/or iso-C_17 : 1ω9c (21.94 %). The prominent isoprenoid quinone was ubiquinone 8 (Q-8). Primary polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and an unknown phospholipid. DNA sequence relatedness between strain CF21T and L. ruishenii CTN-1T was 56 %, which was clearly below the 70 % threshold for prokaryotic species delineation. These analyses indicated that CF21T is a novel member of genus Lysobacter, for which the name Lysobacter chengduensis sp. nov. is proposed. The type strain is CF21T (=CGMCC1.15145T = DSM 100306T). Electronic supplementary materialThe online version of this article (doi:10.​1007/​s00284-015-0921-8) contains supplementary material, which is available to authorized users.