Identification and characterization of a ubiquitin-conjugating enzyme UBE2A gene from lamprey

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• 作者：Liyong Chen ; Fenfang Wu ; Bo Feng
• 关键词：Ubiquitin ; conjugating enzymes ; UBE2A ; Ubiquitination ; Lamprey
• 刊名：Fish Physiology and Biochemistry
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：42
• 期：1
• 页码：313-320
• 全文大小：2,601 KB
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• 作者单位：Liyong Chen (1)
  Fenfang Wu (2)
  Bo Feng (2)
  
  1. Guangdong Province Key Laboratory for Medical Molecular Diagnostics, Dongguan Scientific Research Center, China-America Cancer Research Institute, Guangdong Medical University, Dongguan, 523808, China
  2. State Key Laboratory of Biocontrol, Guangdong Province Key Laboratory for Pharmaceutical Functional Genes, Department of Biochemistry, College of Life Sciences, Sun Yat-Sen (Zhongshan) University, Guangzhou, 510275, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Hydrobiology
  Animal Physiology
  Animal Anatomy, Morphology and Histology
  Animal Biochemistry
  Zoology
  
• 出版者：Springer Netherlands
• ISSN：1573-5168

文摘

Ubiquitin-conjugating enzymes (E2s) play an important role in the mechanism of ubiquitin transfer. Although in most species many of these enzymes share high sequence and structural conservation, their existence and functions in the lamprey remain unknown. In this study, we identified and characterized a ubiquitin-conjugating enzyme (UBE2A)-like gene in lamprey. The gene, designated as LaUBE2A, contained a 456-bp open reading frame encoding a 152-amino acid protein with a typical UBC domain. Real-time PCR assay showed that LaUBE2A was expressed in various tissues of the adult lamprey, with higher levels in the leukocytes and muscle and lower levels in the skin and liver. The high conservation in amino acid sequence between LaUBE2A and UBE2As from Homo sapiens, Mus musculus, Cavia porcellus, and Alligator sinensi implied that the function of LaUBE2A may be similar to that of UBE2A. Keywords Ubiquitin-conjugating enzymes UBE2A Ubiquitination Lamprey