Genomic and transcriptomic analysis of the endophytic fungus Pestalotiopsis fici reveals its lifestyle and high potential for synthesis of natural products

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• 作者：Xiuna Wang (1) (2)
  Xiaoling Zhang (1)
  Ling Liu (1)
  Meichun Xiang (1)
  Wenzhao Wang (1)
  Xiang Sun (1)
  Yongsheng Che (3)
  Liangdong Guo (1)
  Gang Liu (1)
  Liyun Guo (2)
  Chengshu Wang (4)
  Wen-Bing Yin (1)
  Marc Stadler (5)
  Xinyu Zhang (1)
  Xingzhong Liu (1)
  
  1. State Key Laboratory of Mycology ; Institute of Microbiology ; Chinese Academy of Sciences ; Beijing ; China
  2. Department of Plant Pathology ; China Agricultural University ; Beijing ; China
  3. Department of Natural Products Chemistry ; Beijing Institute of Pharmacology & Toxicology ; Beijing ; China
  4. Key Laboratory of Insect Development and Evolutionary Biology ; Institute of Plant Physiology and Ecology ; Shanghai Institutes for Biological Sciences ; Chinese Academy of Sciences ; Shanghai ; China
  5. Department Microbial Drugs ; Helmholtz Centre for Infection Research ; Braunschweig ; Germany
  
• 关键词：Genome ; Endophyte ; Pestalotiopsis fici ; Secondary metabolite
• 刊名：BMC Genomics
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：16
• 期：1
• 全文大小：3,852 KB
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• 刊物主题：Life Sciences, general; Microarrays; Proteomics; Animal Genetics and Genomics; Microbial Genetics and Genomics; Plant Genetics & Genomics;
• 出版者：BioMed Central
• ISSN：1471-2164

文摘

Background In recent years, the genus Pestalotiopsis is receiving increasing attention, not only because of its economic impact as a plant pathogen but also as a commonly isolated endophyte which is an important source of bioactive natural products. Pestalotiopsis fici Steyaert W106-1/CGMCC3.15140 as an endophyte of tea produces numerous novel secondary metabolites, including chloropupukeananin, a derivative of chlorinated pupukeanane that is first discovered in fungi. Some of them might be important as the drug leads for future pharmaceutics. Results Here, we report the genome sequence of the endophytic fungus of tea Pestalotiopsis fici W106-1/CGMCC3.15140. The abundant carbohydrate-active enzymes especially significantly expanding pectinases allow the fungus to utilize the limited intercellular nutrients within the host plants, suggesting adaptation of the fungus to endophytic lifestyle. The P. fici genome encodes a rich set of secondary metabolite synthesis genes, including 27 polyketide synthases (PKSs), 12 non-ribosomal peptide synthases (NRPSs), five dimethylallyl tryptophan synthases, four putative PKS-like enzymes, 15 putative NRPS-like enzymes, 15 terpenoid synthases, seven terpenoid cyclases, seven fatty-acid synthases, and five hybrids of PKS-NRPS. The majority of these core enzymes distributed into 74 secondary metabolite clusters. The putative Diels-Alderase genes have undergone expansion. Conclusion The significant expansion of pectinase encoding genes provides essential insight in the life strategy of endophytes, and richness of gene clusters for secondary metabolites reveals high potential of natural products of endophytic fungi.