Endophytic fungi from a pharmaceutical plant, Camptotheca acuminata: isolation, identification and bioactivity

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• 作者：Xiang Lin ; Chunhua Lu ; Yaojian Huang ; Zhonghui Zheng ; Wenjin Su and Yemao Shen
• 关键词：Endophytic fungi ; Identification ; Bioactivity ; Camptotheca acuminata
• 刊名：World Journal of Microbiology and Biotechnology
• 出版年：2007
• 出版时间：July, 2007
• 年：2007
• 卷：23
• 期：7
• 页码：1037-1040
• 全文大小：172 KB

文摘

About 174 endophytic fungi were isolated from the pharmaceutical plant, Camptotheca acuminata. Of the 18 taxa obtained, non-sporulating fungi (48.9 % ), Alternaria (12.6 % ), Phomopsis (6.9 % ), Sporidesmium (6.3 % ), Paecilomyces (4.6 % ) and Fusarium (4.6 % ) were dominant. ITS rDNA assay indicated that most of the non-sporulating fungi belonged to the Pyrenomycetes and Loculoascomycetes ascomycetes or their anamorph Coelomycetes. The results of the bioactivity test showed that 27.6 % of the endophytic fungi displayed inhibition against more than one indicator microorganism. 4.0 % and 2.3 % of the endophytic fungi showed cytotoxicity and protease inhibition, respectively. The endophytic fungi with bioactivities were distributed in more than 12 taxa including non-sporulating fungi, which are reliable sources for bioactive agents.