Newly discovered colour-pattern polymorphism of Bombus koreanus females (Hymenoptera: Apidae) demonstrated by DNA barcoding

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• 作者：Jiaxing HUANG (1)
  Jie WU (1)
  Jiandong AN (1)
  Paul H. WILLIAMS (1) (2)
  
  1. Key Laboratory for Insect-Pollinator Biology of the Ministry of Agriculture ; Institute of Apiculture ; Chinese Academy of Agricultural Sciences ; No.1 Beigou ; Xiangshan ; Haidian District ; Beijing ; 100093 ; China
  2. Department of Life Sciences ; Natural History Museum ; London ; SW7 5BD ; UK
  
• 关键词：Bombus koreanus ; DNA barcoding ; taxonomy ; colour pattern
• 刊名：Apidologie
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：46
• 期：2
• 页码：250-261
• 全文大小：2,428 KB
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• 刊物主题：Entomology; Life Sciences, general;
• 出版者：Springer Paris
• ISSN：1297-9678

文摘

Bombus (Megabombus) koreanus (Skorikov) is a long-tongued bumblebee that specialises in visiting deep flowers. To describe the distribution, colour-pattern variation and food plants of B. koreanus, we collected specimens and DNA cytochrome oxidase I (COI) barcodes were obtained. Bayesian phylogenetic analysis of barcodes for Megabombus species shows that B. koreanus is one of the more recently diverged species and the sister species to Bombus consobrinus. Our results show that specimens with previously unknown colour patterns also belong to B. koreanus. We describe nine different colour patterns for workers and three for queens, in which the hair of the thorax varies from black to orange-brown. However, there is a lack of covariation between COI and colour-pattern variation. B. koreanus has been recorded to visit 11 species of food plants showing that B. koreanus is polylectic. Our results demonstrate that COI barcoding is an effective method for studying closely related taxa and for recognising species with variable colour patterns.