Silk microfiber-reinforced silk composite scaffolds: fabrication, mechanical properties, and cytocompatibility

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• 作者：Gang Li ; Fei Li ; Zhaozhu Zheng ; Tingting Luo ; Jian Liu…
• 刊名：Journal of Materials Science
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：51
• 期：6
• 页码：3025-3035
• 全文大小：1,509 KB
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• 作者单位：Gang Li (1)
  Fei Li (1)
  Zhaozhu Zheng (1)
  Tingting Luo (1)
  Jian Liu (1)
  Jianbing Wu (1)
  Xiaoqin Wang (1) (2)
  David L. Kaplan (1) (2)
  
  1. National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123, People’s Republic of China
  2. Department of Biomedical Engineering, Tufts University, 4 Colby St, Medford, MA, 02155, USA
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Materials Science
  Characterization and Evaluation Materials
  Polymer Sciences
  Continuum Mechanics and Mechanics of Materials
  Crystallography
  Mechanics
  
• 出版者：Springer Netherlands
• ISSN：1573-4803

文摘

Mechanical reinforcement of silk membranes by embedding silk microfibers was studied. Silk microfibers of 10–600 μm long were prepared by hydrolyzing degummed silk fibers in alkali solution. The silk microfibers were mixed with silk fibroin solution (continuous phase) at various ratios to fabricate silk microfiber-reinforced composite scaffolds (SMCSs). The morphology, mechanical properties, structural characteristics, biodegradation, and cytotoxicity of the composites were investigated. Silk microfiber-reinforced membranes with 1 wt% of microfibers displayed the most homogeneous distribution of microfibers in the membrane matrix. The tensile strength and elongation at break were 10.5 ± 2.7 MPa and 56.7 ± 7.8 %, respectively, comparable to human meniscus tissue. The presence of silk microfibers did not significantly impact the secondary structure and crystallization of SMCSs. Proteolytic degradation in vitro using protease XIV showed that the 1 % silk microfiber-reinforced membranes lost 90 % weight after 5 days, a longer time frame than plain silk membrane controls. The viability of human fibroblasts (Hs 865.Sk) on the SMCSs demonstrated no cytotoxicity. SMCSs may be useful as biomaterial systems as tissue substitutes where mechanical strength is critical for functional performance. Gang Li and Fei Li have contributed to this manuscript equally.