A toxicological study of inhalable particulates by plasmid DNA assay: A case study from Macao
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- 作者：LongYi Shao (1)
RongRong Shen (1)
Jing Wang (1)
ZhiShi Wang (2)
Uwa Tang (2)
ShuShen Yang (3) - 关键词：inhalable particulate matter (PM10) ; oxidative damage ; heavy metals ; Macao
- 刊名：Science China Earth Sciences
- 出版年：2013
- 出版时间：June 2013
- 年：2013
- 卷：56
- 期：6
- 页码：1037-1043
- 全文大小：524KB
- 参考文献：1. Ostro B, Broadwin R, Green S, et al. Fine particulate air pollution and mortality in nine California counties: Result from CALFINE. Environ Health Prosp, 2006, 114: 29-3 CrossRef
2. Lin Z Q, Xi Z G, Chao F H. Research progress in the toxicity effects of the nano particles. J Prev Med Chin PLA, 2007, 25: 383-86
3. Zhang W L, Xu D Q, Cui J S. Air pollutant PM2.5 monitoring and study on its genotoxicity. J Environ Health, 2003, 1: 3-
4. Shao L Y, Li J J, Zhao H Y, et al. Associations between particle physicochemical characteristics and oxidative capacity: An indoor PM10 study in Beijing, China. Atmos Environ, 2007, 41: 5316-326 CrossRef
5. Li X Y, Gilmour P S, Donaldson K, et al. / In vivo and / in vitro proinflammatory effects of particulate air pollution (PM10). Environ Health Persp, 1997, 105: 1279-283
6. Greenwell L L, Moreno T, Richard R J. Pulmonary antioxidants exert differential protective effects against urban and industrial particulate matter. J Biosci, 2003, 28: 101-07 CrossRef
7. Costa D L, Dreher K L. Bioavailable transition metals in particulate matter mediate cardiopulmonary injury in healthy and compromised animal models. Environ Health Persp, 1997, 105: 1053-060
8. Shi Z B, Shao L Y, Jones T P, et al. The plasmid DNA oxidative damage by the respirable particulates in the urban atmosphere. Chin Sci Bull, 2004, 49: 673-78
9. Whittaker A G. Black smokes: Past and present. The Thesis for the Degree of Ph.D. Cardiff University, 2003
10. Shao L Y, Yang S S, Shi Z B, et al. Physicochemical Characteristics and Bioreactivity Research of the Respirable Particulates in the Urban Atmosphere. Beijing: Meteorological Press, 2006. 1-09
11. Wang Z S. Urban traffic pollution simulation in Macao peninsula. Urban Devel Res, 2003, 20: 15-7
12. China Statistic Department. China Statistics Yearbook. Beijing: China Statistics Press, 2008
13. Lv S L, Shao L Y, Wu M H, et al. Correlation between plasmid DNA damage induced by PM10 and trace metals in inhalable particulate matters in Beijing air. Sci China Ser D-Earth Sci, 2006, 49: 1323-331 CrossRef
14. Moreno T, Merolla L, Gibbons W, et al. Variations in the source, metal content and bioreactivity of technogenic aerosols: A case study from Port Talbot, Wales, UK. Sci Total Environ, 2004, 333: 59-3 CrossRef
15. Shen R R, Shao L Y, Wang Z S, et al. A toxicological study based on DNA damage of inhalable particulates collected in winter in Macao. Chin Environ Sci, 2009, 29: 991-96
16. Adamson I Y R, Prieditis H, Vincent R. Pulmonary toxicity of an atmospheric particulate sample is due to the soluble fraction. Toxicol Appl Pharm, 1999, 157: 43-0 CrossRef
17. Adamson I Y R, Prieditis H, Hedgecock C, et al. Zinc is the toxic factor in the lung response to an atmospheric particulate sample. Toxicol Appl Pharm, 2000, 166: 111-19 CrossRef
18. Shao L Y, Shi Z B, Jones T P, et al. Bioreactivity of particulate matter in Beijing air: Results from plasmid DNA assay. Sci Total Environ, 2006, 367: 261-72 CrossRef
19. Richards R J, Atkins J, Marrs T C, et al. The biochemical and pathological changes produced by the intratracheal instillation of certain components of zinc-hexachloroethane smoke. Toxicol Lett, 1989, 54: 79-8 CrossRef - 作者单位：LongYi Shao (1)
RongRong Shen (1)
Jing Wang (1)
ZhiShi Wang (2)
Uwa Tang (2)
ShuShen Yang (3)
1. State Key Laboratory of Coal Resources and Safe Mining, and College of Geosciences and Surveying Engineering, China University of Mining and Technology, Beijing, 100083, China
2. Faculty of Science and Technology, University of Macao, Macao, 000853, China
3. Zhongyuan University of Technology, Zhengzhou, 450007, China - ISSN：1869-1897
文摘
Oxidative damage to plasmid DNA induced by airborne PM10 (particulate matter with an aerodynamic diameter of 10 μm or less) is caused by the bioavailable (i.e., soluble) heavy metals on the particle surface. However, quantitative analyses of the links between PM10 and oxidative damage are limited. In this study, plasmid DNA assay and ICP-MS were applied to study oxidative capacity and trace element compositions, respectively, of summer and winter PM10 samples collected at several sites (Sun Yat Sen Municipal Park (SYSP) and Av. de Horta e Costa (AHC) on the Macao peninsula and Macao University on Taipa Island (TI)) in Macao. At AHC and TI, the oxidative capacity of PM10 collected in winter was higher than that collected in summer, for both the whole sample and the water-soluble fraction. In contrast, no seasonal variation was noted at SYSP. PM10 exhibited the highest oxidative capacity at SYSP and lowest oxidative capacity at TI in both seasons, demonstrating that the PM10 collected on the Macao peninsula had a higher toxicity than that from Taipa Island. ICP-MS analyses revealed that the concentrations of total analyzed trace elements and their water-soluble components in PM10 from TI and AHC were higher in winter than in summer, whereas SYSP displayed the opposite trend. The extents of oxidative damage induced by the water-soluble fractions and intact whole particles were generally similar, implying that the oxidative damage caused by particles in Macao resulted mainly from the water-soluble fraction. The oxidative capacities of PM10 were positively correlated with both whole and soluble Zn at the 95% confidence level, indicating that Zn was the major element responsible for the oxidative damage caused by particles in Macao. Other heavy metals, such as Cr, Cu, Cd, Ni, As, and Pb, also exhibited elevated concentrations, and the potential health impacts of these metals should be considered.