5-farnesyloxycoumarin: a potent 15-LOX-1 inhibitor, prevents prostate cancer cell growth

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• 作者：Ala Orafaie ; Hamid Sadeghian ; Ahmad Reza Bahrami…
• 关键词：5 ; farnesyloxycoumarin ; 15 ; LOX ; 1 ; PC3 ; DU145
• 刊名：Medicinal Chemistry Research
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：26
• 期：1
• 页码：227-234
• 全文大小：
• 刊物主题：Pharmacology/Toxicology; Biochemistry, general; Cell Biology;
• 出版者：Springer US
• ISSN：1554-8120
• 卷排序：26

文摘

In this study, the activity of 15-LOX-1 was first evaluated in two prostate cancer cell lines PC3 and DU145. Considering the enzyme inhibitory potency of 5-farnesyloxycoumarin (5f), the cytotoxic effects of this compound was studied in PC3, DU145 and HFF3 (normal cell line) by MTT assay. Since 5f was more effective on PC3 cells, this cell line was used for further explorations. The chromatin condensation and DNA damage were studied by DAPI staining and comet assay, respectively and cell cycle analysis was performed by propidium iodide staining. Results indicated that 15-LOX-1 activity was very low in DU145 cells. Moreover, PC3 cells were more sensitive to 5f as compared to DU145 cancerous cells, while no significant effect was observed on normal HFF3 cells. Interestingly, the IC₅₀ values of 5f on PC3 cells were similar to cisplatin. DAPI staining showed that 5f induced chromatin condensation in 63 % of PC3 cells. Comet assay also demonstrated 53 % “DNA in tail” for PC3 cells. Finally, 5f induced G₀/G₁ arrest in PC3 cell cycle. These findings suggest that cytotoxic effects of 5f might be due to the inhibition of 15-LOX-1 in PC3 cell line and, it can be introduced as a potent anticancer compound.