Validation and identification of molecular markers linked to the leaf rust resistance gene Lr28 in wheat

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• 作者：Annapurnalilly Bipinraj (1)
  Balgounda Honrao (1)
  Mohinder Prashar (2)
  Subhash Bhardwaj (2)
  Suryaprakasa Rao (1)
  Shubhada Tamhankar (1)
  
• 关键词：Lr28 ; Marker assisted selection ; Microsatellite markers ; Puccinia triticina ; Triticum aestivum
• 刊名：Journal of Applied Genetics
• 出版年：2011
• 出版时间：May 2011
• 年：2011
• 卷：52
• 期：2
• 页码：171-175
• 全文大小：175KB
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• 作者单位：Annapurnalilly Bipinraj (1)
  Balgounda Honrao (1)
  Mohinder Prashar (2)
  Subhash Bhardwaj (2)
  Suryaprakasa Rao (1)
  Shubhada Tamhankar (1)
  
  1. Genetics and Plant Breeding Group, Agharkar Research Institute, G. G. Agarkar Road, Pune, 411 004, India
  2. Directorate of Wheat Research, Regional Station, Flowerdale, Shimla, 171 002, India
  
• ISSN：2190-3883

文摘

Leaf rust resistance gene Lr28 controls one of the important resistances in the Indian subcontinent against the most prevalent Puccinia triticina pathotype 77-5. Pyramiding Lr28 with other resistance genes would therefore, provide durable resistance against rust, a process that can be facilitated by DNA markers. A microsatellite marker wmc313 linked to Lr28 at a distance of 5.0?cM was identified in the population HD2329 × HW2037. The marker was validated in another population developed from WL711× CS + Lr28: 2D/2M 3/8 (acc. 2956) as well as in a few near- isogenic lines (NILs) carrying gene Lr28. Compared to the previously reported marker TPSCAR SCS421570, wmc313 is more closely linked to Lr28. Both these markers flanking the gene should be useful in the deployment of Lr28 into the breeding program using marker-assisted selection allowing pyramiding with other effective genes to confer durable resistance.