Exopolysaccharide production in Anabaena sp. PCC 7120 under different CaCl2 regimes
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- 作者：Savita Singh ; Ekta Verma ; Niveshika…
- 关键词：Anabaena 7120 ; Extracellular polymeric substances ; Fourier transform infra red spectroscopy ; Quantitative reverse transcriptase ; polymerase chain reaction ; alr2882 gene ; Scanning electron microscopy
- 刊名：Physiology and Molecular Biology of Plants
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：22
- 期：4
- 页码：557-566
- 全文大小：
- 刊物主题：Plant Sciences; Plant Physiology; Biological and Medical Physics, Biophysics; Cell Biology;
- 出版者：Springer India
- ISSN：0974-0430
- 卷排序：22
文摘
Influence of various levels of CaCl2 (0, 1, 10 and 100 mM) on exopolysaccharide production has been investigated in the cyanobacterium Anabaena 7120. At the concentration of 1 mM CaCl2, growth was found to be stimulatory while 100 mM was sub lethal for the cyanobacterial cells. Estimation of EPS content revealed that EPS production depends on the concentration of calcium ions in the immediate environment with maximum being at10 mM CaCl2. A possible involvement of alr2882 gene in the process of EPS production was also revealed through qRT-PCR. Further, FTIR-spectra marked the presence of aliphatic alkyl-group, primary amine-group, and polysaccharides along with shift in major absorption peaks suggesting that calcium levels in the external environment regulate the composition of EPS produced by Anabaena 7120. Thus, both quantity and composition of EPS is affected under different calcium chloride concentrations presenting possibilities of EPS with novel unexplored features that may offer biotechnological applications.