LSM14A inhibits porcine reproductive and respiratory syndrome virus (PRRSV) replication by activating IFN-β signaling pathway in Marc-145

详细信息    查看全文

• 作者：Zhenhong Li (1)
  Rui Chen (2)
  Jinhua Zhao (1)
  Ziyu Qi (1)
  Likai Ji (1)
  Yueran Zhen (1)
  Bang Liu (1)
  
• 关键词：LSM14A ; PRRSV ; PRRs ; IFN ; β ; Antiviral ; Marc ; 145 cells ; PAMs
• 刊名：Molecular and Cellular Biochemistry
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：399
• 期：1-2
• 页码：247-256
• 全文大小：2,514 KB
• 参考文献：1. Snijder EJ, Meulenberg JJM (1998) The molecular biology of arteriviruses. J Gen Virol 79:961-79
  2. Allende R, Lewis TL, Lu Z, Rock DL, Kutish GF, Ali A, Doster AR, Osorio FA (1999) North American and European porcine reproductive and respiratory syndrome viruses differ in non-structural protein coding regions. J Gen Virol 80:307-15
  3. Meulenberg JJ (2000) PRRSV, the virus. Vet Res 31:11-1
  4. Rossow KD (1998) Porcine reproductive and respiratory syndrome. Vet Pathol 35:1-0 CrossRef
  5. Holtkamp D, Kliebenstein J (2011) PRRS Costs industry $664 million annually. Pork Checkoff Study [http://www.pork.org/News/1265/PRRSCostsIndustry 664 Million.aspx]
  6. Chang CC, Yoon KJ, Zimmerman JJ, Harmon KM, Dixon PM, Dvorak CM, Murtaugh MP (2002) Evolution of porcine reproductive and respiratory syndrome virus during sequential passages in pigs. J Virol 76:4750-763 CrossRef
  7. Drew TW (2000) A review of evidence for immunosuppression due to porcine reproductive and respiratory syndrome virus. Vet Res 31:27-9
  8. Kawai T, Takahashi K, Sato S, Coban C, Kumar H, Kato H, Ishii KJ, Takeuchi O, Akira S (2005) IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction. Nat Immunol 6:981-88 CrossRef
  9. Miller LC, Laegreid WW, Bono JL, Chitko-McKown CG, Fox JM (2004) Interferon type I in porcine reproductive and respiratory syndrome virus-infected Marc-145 cells. Arch Virol 149:2453-463 CrossRef
  10. Murtaugh MP, Xiao Z, Zuckermann F (2002) Immunological responses of swine to porcine reproductive and respiratory syndrome virus infection. Viral Immunol 15:533-47 CrossRef
  11. Luo R, Xiao S, Jiang Y, Jin H, Wang D, Liu M, Chen H, Fang L (2008) Porcine reproductive and respiratory syndrome virus (PRRSV) suppresses interferon-beta production by interfering with the RIG-I signaling pathway. Mol Immunol 45:2839-846 CrossRef
  12. Akira S, Uematsu S, Takeuchi O (2006) Pathogen recognition and innate immunity. Cell 124:783-01 CrossRef
  13. Pichlmair A, Reise Sousa C (2007) Innate recognition of viruses. Immunity 27:370-83 CrossRef
  14. Barbalat R, Ewald SE, Mouchess ML, Barton GM (2011) Nucleic acid recognition by the innate immune system. Annu Rev Immunol 29:185-14 CrossRef
  15. Kawai T, Akira S (2011) Toll-like receptors and their crosstalk with other innate receptors in infection and immunity. Immunity 34:637-50 CrossRef
  16. Unterholzner L, Keating SE, Baranl M, Horan KA, Jensen SB, Sharma S, Sirois CM, Jin T, Latz E, Xiao TS, Fitzgerald KA, Paludan SR, Bowiel AG (2010) IFI16 is an innate immune sensor for intracellular DNA. Nat Immunol 11:997-004 CrossRef
  17. Zhang ZQ, Yuan B, Bao MS, Lu N, Kim T, Liu YJ (2011) The helicase DDX41 senses intracellular DNA mediated by the adaptor STING in dendritic cells. Nat Immunol 12:959-65 CrossRef
  18. Li Y, Chen R, Zhou Q, Xu Z, Li C, Wang S, Mao A, Zhang X, He W, Shu HB (2012) LSm14A is a processing body-associated sensor of viral nucleic acids that initiates cellular antiviral response in the early phase of viral infection. Proc Natl Acad Sci 109:11770-1775 CrossRef
  19. Li B, Xiao S, Wang Y, Xu S, Jiang Y, Chen H, Fang L (2009) Immunogenicity of the highly pathogenic porcine reproductive and respiratory syndrome virus GP5 protein encoded by a
• 作者单位：Zhenhong Li (1)
  Rui Chen (2)
  Jinhua Zhao (1)
  Ziyu Qi (1)
  Likai Ji (1)
  Yueran Zhen (1)
  Bang Liu (1)
  
  1. Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education and College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China
  2. Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC, 27710, USA
  
• ISSN：1573-4919

文摘

Porcine reproductive and respiratory syndrome virus (PRRSV) is considered as a significant contributor to porcine reproductive and respiratory syndrome, one of the most economically important diseases for the pig industry worldwide. Emerging evidence indicates that pattern recognition receptors play key roles in recognizing pathogen-associated molecular patterns. In the present study, we investigated the effects of a novel pattern recognition receptor LSM14A in regulating PRRSV replication. Results in Marc-145 cells and porcine alveolar macrophages (PAMs) indicated that overexpression of porcine LSM14A effectively inhibited the replication of PRRSV, and knockdown of LSM14A by siRNA enhanced the replication of PRRSV. Mechanistically, LSM14A up-regulated the activities of IFN-β and ISRE promoters, enhanced the production of IFN-β, RIG-I, and ISGs, and inhibited the production of the inflammatory cytokines of TNF-α and IL-6 mRNA. Additionally, the expression pattern of LSM14A during the infection of PRRSV in Tongcheng and Large White pigs was suppressed by the PRRSV challenge. Taken together, our results suggest that LSM14A is an important PRR that inhibits PPRSV replication by inducing IFN-β signaling and restraining inflammatory responses. Furthermore, the down-regulation of LSM14A by PRRSV might represent an important mechanism by which PRRSV invades the host. Our study sheds light on the possibility of developing a new strategy to control this disease.