Co-localization of LTBP-2 with FGF-2 in fibrotic human keloid and hypertrophic scar

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• 作者：Mohamed A. Sideek ; Abdulrahman Teia ; Zlatko Kopecki…
• 关键词：LTBP ; 2 ; FGF ; 2 ; Fibrosis ; Keloid ; Hypertrophic scar
• 刊名：Journal of Molecular Histology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：47
• 期：1
• 页码：35-45
• 全文大小：2,718 KB
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• 作者单位：Mohamed A. Sideek (1) (2)
  Abdulrahman Teia (1)
  Zlatko Kopecki (3)
  Allison J. Cowin (3)
  Mark A. Gibson (1)
  
  1. Discipline of Anatomy and Pathology, School of Medicine, University of Adelaide, Adelaide, SA, 5005, Australia
  2. Department of Biomedical Sciences, Kulliyyah of Allied Health Sciences, International Islamic University Malaysia, 25200, Kuantan, Pahang, Malaysia
  3. Regenerative Medicine, Mawson Institute, University of South Australia, Adelaide, SA, 5095, Australia
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Biomedicine
  Developmental Biology
  
• 出版者：Springer Netherlands
• ISSN：1567-2387

文摘

We have recently shown that Latent transforming growth factor-beta-1 binding protein-2 (LTBP-2) has a single high-affinity binding site for fibroblast growth factor-2 (FGF-2) and that LTBP-2 blocks FGF-2 induced cell proliferation. Both proteins showed strong co-localisation within keloid skin from a single patient. In the current study, using confocal microscopy, we have investigated the distribution of the two proteins in normal and fibrotic skin samples including normal scar tissue, hypertrophic scars and keloids from multiple patients. Consistently, little staining for either protein was detected in normal adult skin and normal scar samples but extensive co-localisation of the two proteins was observed in multiple examples of hypertrophic scars and keloids. LTBP-2 and FGF-2 were co-localised to fine fibrous elements within the extracellular matrix identified as elastic fibres by immunostaining with anti-fibrillin-1 and anti-elastin antibodies. Furthermore, qPCR analysis of RNA samples from multiple patients confirmed dramatically increased expression of LTBP-2 and FGF-2, similar TGF-beta 1, in hypertrophic scar compared to normal skin and scar tissue. Overall the results suggest that elevated LTBP-2 may bind and sequester FGF-2 on elastic fibres in fibrotic tissues and modulate FGF-2’s influence on the repair and healing processes. Keywords LTBP-2 FGF-2 Fibrosis Keloid Hypertrophic scar