Determination of Pesticide Residues in Golden Berry (Physalis peruviana L.) by Modified QuEChERS Method and Ultra-High Performance Liquid Chromatography-Tandem Quadrupole Mass Spectrometry

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• 作者：Natalia Cadavid Muñoz ; Luana Floriano ; Maiara P. de Souza…
• 关键词：Pesticides ; Golden berry ; Food ; QuEChERS ; UHPLC ; MS/MS
• 刊名：Food Analytical Methods
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：10
• 期：2
• 页码：320-329
• 全文大小：
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Food Science; Chemistry/Food Science, general; Microbiology; Analytical Chemistry;
• 出版者：Springer US
• ISSN：1936-976X
• 卷排序：10

文摘

A fast and effective multiresidue method for the determination of 42 pesticides in golden berry was developed and validated. A modified QuEChERS method was established for sample preparation followed by ultra-high performance liquid chromatographic-tandem mass spectrometry (UHPLC-MS/MS) determination with electrospray ionization in a triple quadrupole system. Validation results were satisfactory, since the method presented recoveries between 70 and 114 % with relative standard deviations (RSD) <20 % for blank samples spiked from 5 to 25 μg kg−1. The method limit of detection and limit of quantification were 1.5 and 5 μg kg−1_, respectively. Matrix effect ranged from −32 to 218 % and was compensated using matrix-matched calibration. Method linearity was established from 2.5 to 100 μg kg−1 with r2 ≥ 0.99. The proposed method combines the advantages of a simple and fast sample preparation step by a modified QuEChERS method with the high selectivity and sensitivity of the UHPLC-MS/MS system using selected reaction monitoring. The method was successfully applied to commercial samples, proving to be an efficient alternative for routine analysis. From the 16 analyzed samples, 13 presented residues of one or more pesticides (carbendazim, chlorpyrifos ethyl, dimethoate, propamocarb, and tebuconazole) in the concentration range of 2.0 to 55.6 μg kg−1.