GADD45&ggr;, down-regulated in 65% Hepatocellular Carcinoma (HCC) from 23 Chinese patients, inhibits cell growth and induces cell cycle G2/M arrest for Hepatoma Hep-G2 cell lines

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• 作者：Sun ; Luhong ; Gong ; Ruomu ; Wan ; Bo ; Huang ; Xinghua ; Wu ; Chaoqun ; Zhang ; Xirang ; Zhao ; Shouyuan ; Yu ; Long
• 关键词：colony formation ; gene expression ; growth inhibition
• 刊名：Molecular Biology Reports
• 出版年：2003
• 出版时间：2003
• 年：2003
• 卷：30
• 期：4
• 页码：249-253
• 全文大小：145 KB

文摘

Growth-arrest and DNA-damage inducible (GADD) genes and Myeloid differentiation primary response (MyD) genes represent a family of genes that play a key role in negative control of cell growth. In the present study, following clone and location of human GADD45 &ggr; (MyDL) gene, we have found that its mRNA expression level was down-regulated in 15/23 cases of clinic hepatocellular carcinoma (HCC) by comparing the northern hybridization results between the tumor tissues and adjacent normal tissues. Transient transfection of GADD45 &ggr; cDNA with intact open reading frame sequence into the human hepatoma cells Hep-G2 resulted in dramatic growth suppression in colony formation assays. Furthermore, flow cytometry analysis indicated that GADD45 &ggr; caused cell cycle arrest at G2/M transition when transfected into Hep-G2 cells. Therefore, the possible role of GADD45 &ggr; in cell growth control was further confirmed in this paper.