Proteomic analysis of Mortierella isabellina M6-22 during cold stress
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- 作者：Binbin Hu ; Minzhou Luo ; Xiuling Ji ; Lianbing Lin ; Yunlin Wei…
- 关键词：Mortierella isabellina ; Cold stress ; Proteomics ; Membrane fluidity
- 刊名：Archives of Microbiology
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：198
- 期：9
- 页码：869-876
- 全文大小：1,391 KB
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Microbiology
Microbial Ecology
Biochemistry
Cell Biology
Biotechnology
Ecology - 出版者：Springer Berlin / Heidelberg
- ISSN：1432-072X
- 卷排序：198
文摘
We aimed to gain a better understanding of cold adaption in Mortierella isabellina M6-22 by using proteomics approaches. The temperature range and optimal temperature for M6-22 growth were investigated, and composition changes in fatty acids were analyzed. Accompanied with the 2-D gel electrophoresis, MALDI-TOF/TOF–MS analysis was conducted to characterize alterations in protein profiling in M6-22 cultured at 30 °C for 24 h and 15 °C for another 24 h when compared with those cultured at 30 °C for 48 h. Gene Ontology (GO) cluster analysis was finally conducted for successfully identified proteins. M6-22 cells could grow well at temperatures ranging from 15 to 30 °C. As temperature decreased from 30 to 15 °C, LA and GLA significantly increased from 11.63 to 17.85 % and from 9.12 to 13.19 %, respectively, while oleic acid significantly decreased from 47.25 to 36.53 %. Proteomics analyses revealed 111 differentially expressed protein spots, among which 5 unique proteins (A38, A40, A47, A49 and A58), 29 up-regulated proteins and 10 down-regulated proteins were identified by MALDI-TOF/TOF–MS. GO enrichment analysis demonstrated that these proteins mainly involved in glycolytic pathway (A34 and A50), electron transport (A28), ATP production (A35 and B39) and protein modification (A38). A total of 44 differentially expressed proteins have been successfully identified in M. isabellina M6-22 cultured at 15 °C. These proteins may play important roles in cold adaption via regulation of ATP synthesis, activation of cold-adaptive proteins, degradation of needless protein, accumulation of PUFAs, etc.