Microbial production strategies and applications of lycopene and other terpenoids

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• 作者：Tian Ma ; Zixin Deng ; Tiangang Liu
• 关键词：Lycopene ; Metabolic engineering ; Biosynthesis ; Synthetic biology ; Terpenoid
• 刊名：World Journal of Microbiology & Biotechnology
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：32
• 期：1
• 全文大小：433 KB
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• 作者单位：Tian Ma (1)
  Zixin Deng (1) (2)
  Tiangang Liu (1) (2) (3)
  
  1. Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, Wuhan University School of Pharmaceutical Sciences, Donghu Road, Wuhan, 430071, People’s Republic of China
  2. Hubei Engineering Laboratory for Synthetic Microbiology, Wuhan Institute of Biotechnology, Wuhan, People’s Republic of China
  3. Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Wuhan, People’s Republic of China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Applied Microbiology
  Biotechnology
  Biochemistry
  Environmental Biotechnology
  Microbiology
  
• 出版者：Springer Netherlands
• ISSN：1573-0972

文摘

Terpenoids are a large class of compounds that have far-reaching applications and economic value, particularly those most commonly found in plants; however, the extraction and synthesis of these compounds is often expensive and technically challenging. Recent advances in microbial metabolic engineering comprise a breakthrough that may enable the efficient, cost-effective production of these limited natural resources. Via the engineering of safe, industrial microorganisms that encode product-specific enzymes, and even entire metabolic pathways of interest, microbial-derived semisynthetic terpenoids may soon replace plant-derived terpenoids as the primary source of these valuable compounds. Indeed, the recent metabolic engineering of an Escherichia coli strain that produces the precursor to lycopene, a commercially and medically important compound, with higher yields than those in tomato plants serves as a successful example. Here, we review the recent developments in the metabolic engineering of microbes for the production of certain terpenoid compounds, particularly lycopene, which has been increasingly used in pharmaceuticals, nutritional supplements, and cosmetics. Furthermore, we summarize the metabolic engineering strategies used to achieve successful microbial production of some similar compounds. Based on this overview, there is a reason to believe that metabolic engineering comprises an optimal approach for increasing the production of lycopene and other terpenoids.