Preparing the lethal hit: interplay between exo- and endocytic pathways in cytotoxic T lymphocytes
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- 作者：Hsin-Fang Chang ; Hawraa Bzeih ; Praneeth Chitirala…
- 关键词：Correlative light and electron microscopy ; Early endosomes ; Late endosomes ; Recycling endosomes ; SNARE proteins ; Total internal reflection fluorescence microscopy
- 刊名：Cellular and Molecular Life Sciences
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：74
- 期：3
- 页码：399-408
- 全文大小：869KB
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Cell Biology; Biomedicine, general; Life Sciences, general; Biochemistry, general;
- 出版者：Springer International Publishing
- ISSN：1420-9071
- 卷排序：74
文摘
Cytotoxic T lymphocytes patrol our body in search for infected cells which they kill through the release of cytotoxic substances contained in cytotoxic granules. The fusion of cytotoxic granules occurs at a specially formed contact site, the immunological synapse, and is tightly controlled to ensure specificity. In this review, we discuss the contribution of two intracellular compartments, endosomes and cytotoxic granules, to the formation, function and disassembly of the immunological synapse. We highlight a recently proposed sequential process of fusion events at the IS upon target cell recognition. First, recycling endosomes fuse with the plasma membrane to deliver cargo required for the docking of cytotoxic granules. Second, cytotoxic granules arrive and fuse upon docking in a SNARE-dependent manner. Following fusion, membrane components of the cytotoxic granule are retrieved through endocytosis to ensure the fast, efficient serial killing of target cells that is characteristic of cytotoxic T lymphocytes.